Recombinant Saccharomyces cerevisiae Kinetochore-associated protein CNN1 (CNN1)

Code CSB-YP331894SVG
MSDS
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Source Yeast
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Code CSB-EP331894SVG
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Source E.coli
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Code CSB-EP331894SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP331894SVG
MSDS
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Source Baculovirus
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Code CSB-MP331894SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CNN1
Uniprot No.
Alternative Names
CNN1; YFR046C; Inner kinetochore subunit CNN1; CENP-T homolog; Co-purified with NNF1 protein 1; Constitutive centromere-associated network protein CNN1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-361
Target Protein Sequence
MSTPRKAAGN NENTEVSEIR TPFRERALEE QRLKDEVLIR NTPGYRKLLS ASTKSHDILN KDPNEVRSFL QDLSQVLARK SQGNDTTTNK TQARNLIDEL AYEESQPEEN ELLRSRSEKL TDNNIGNETQ PDYTSLSQTV FAKLQERDKG LKSRKIDPII IQDVPTTGHE DELTVHSPDK ANSISMEVLR TSPSIGMDQV DEPPVRDPVP ISITQQEEPL SEDLPSDDKE ETEEAENEDY SFENTSDENL DDIGNDPIRL NVPAVRRSSI KPLQIMDLKH LTRQFLNENR IILPKQTWST IQEESLNIMD FLKQKIGTLQ KQELVDSFID MGIINNVDDM FELAHELLPL ELQSRIESYL F
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the kinetochore, a multiprotein complex that assembles on centromeric DNA and attaches chromosomes to spindle microtubules, mediating chromosome segregation and sister chromatid segregation during meiosis and mitosis. Component of the inner kinetochore constitutive centromere-associated network (CCAN), which serves as a structural platform for outer kinetochore assembly. CNN1 is important for the recruitment of the outer kinetochore Ndc80 complex.
Gene References into Functions
  1. Phosphorylation of the Cnn1 N-terminus coordinates the binding of the two competing Ndc80 interaction partners. PMID: 23334295
  2. Cnn1 regulates KMN activity in a spatiotemporal manner by inhibiting the interaction between its complexes PMID: 22561345
  3. Data show that cells that fail to activate Cdk1 are devoid of spindles due to persistently active APCCdh1, which targets microtubule-associated proteins Cin8, Kip1 and Ase1 for degradation. PMID: 16688214
Subcellular Location
Nucleus. Chromosome, centromere, kinetochore.
Protein Families
CENP-T/CNN1 family
Database Links

KEGG: sce:YFR046C

STRING: 4932.YFR046C

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