Recombinant Saccharomyces cerevisiae LAS seventeen-binding protein 5 (LSB5)

Code CSB-YP329545SVG
MSDS
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Source Yeast
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Code CSB-EP329545SVG
MSDS
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Source E.coli
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Code CSB-EP329545SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP329545SVG
MSDS
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Source Baculovirus
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Code CSB-MP329545SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LSB5
Uniprot No.
Alternative Names
LSB5; YCL034W; YCL186; YCL34W; LAS seventeen-binding protein 5; LAS17-binding protein 5
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-354
Target Protein Sequence
MGFLSDHPHT AITETIFRIV SSRDYTLEVE LAPLIQLIKA DHNDYNYTVN QEEAARALRK KIKYGNRLQQ SRTLDLLDLF ISQGVKFTVM YNDDKLLQRL RGMATNSENS GSGEKYEPRI IKKCAAYAIS WLNYITQNNL ENARAYSGLY QLGQTVKQRY SKSSRSRRSG GGSGGRSNFM DDSADDTLYQ SNSLTSADRL YRIPQINMNK EAPRIRLIIS DALASAVSLQ NSLIGLPKGK FSTDDEEATS KFIQARAIRR KVLRYLQLVT EGEFLGSLIH ANDELVAALT AYDDRSAQDD SSDESDHGSY DDGIYDENEQ DNSRYIDSES SEEESLSSYQ PSTISNPFGD HNKI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Essential for the organization of the actin cytoskeleton, fluid phase endocytosis and vesicle trafficking, together with YSC84.
Gene References into Functions
  1. This study describes the role of Lsb5p in membrane-trafficking events at the plasma membrane and also demonstrate for the first time an interaction of Arf3 with the endocytic machinery in yeast. PMID: 15651983
  2. Results discuss the interaction of Sla1p with Lsb5p, and demonstrate a requirement for Arf3p expression in order to localize Lsb5p to the cell cortex. PMID: 16246096
Subcellular Location
Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Note=Localizes independently of F-actin.
Protein Families
LSB5 family
Database Links

KEGG: sce:YCL034W

STRING: 4932.YCL034W

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