Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
ERG11; CYP51; YHR007CLanosterol 14-alpha demethylase; EC 1.14.14.154; CYPLI; Cytochrome P450 51; Cytochrome P450-14DM; Cytochrome P450-LIA1; Sterol 14-alpha demethylase
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Target Protein Sequence
MSATKSIVGEALEYVNIGLS
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Extracellular Domain
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant Baker's yeast ERG11 was expressed with the amino acid range of 1-20. This ERG11 protein is expected to have a theoretical molecular weight of 29.1 kDa. This ERG11 recombinant protein is manufactured in e.coli. The ERG11 gene fragment has been modified by fusing the N-terminal GST tag, providing convenience in detecting and purifying the recombinant ERG11 protein during the following stages.
In Saccharomyces cerevisiae, lanosterol 14-alpha demethylase (ERG11) is a cytochrome P450 monooxygenase that plays a crucial role in the ergosterol biosynthesis pathway. ERG11 catalyzes the demethylation of lanosterol, a key step in the conversion of lanosterol to ergosterol. Ergosterol is the fungal counterpart to mammalian cholesterol and is essential for maintaining the integrity and fluidity of the yeast cell membrane. Inhibition of ERG11 disrupts ergosterol synthesis, leading to impaired membrane function and increased cell permeability. Research areas related to ERG11 include antifungal drug development, as this enzyme is the target of azole antifungal drugs. Understanding the structure and function of ERG11 is crucial for developing new antifungal therapies and combating fungal infections.
