Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ERG11

Uniprot No.

P10614

Research Area

Others

Alternative Names

ERG11; CYP51; YHR007CLanosterol 14-alpha demethylase; EC 1.14.14.154; CYPLI; Cytochrome P450 51; Cytochrome P450-14DM; Cytochrome P450-LIA1; Sterol 14-alpha demethylase

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Source

E.coli

Expression Region

1-20aa

Target Protein Sequence

MSATKSIVGEALEYVNIGLS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

29.1kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Baker's yeast ERG11 was expressed with the amino acid range of 1-20. This ERG11 protein is expected to have a theoretical molecular weight of 29.1 kDa. This ERG11 recombinant protein is manufactured in e.coli. The ERG11 gene fragment has been modified by fusing the N-terminal GST tag, providing convenience in detecting and purifying the recombinant ERG11 protein during the following stages.

In Saccharomyces cerevisiae, lanosterol 14-alpha demethylase (ERG11) is a cytochrome P450 monooxygenase that plays a crucial role in the ergosterol biosynthesis pathway. ERG11 catalyzes the demethylation of lanosterol, a key step in the conversion of lanosterol to ergosterol. Ergosterol is the fungal counterpart to mammalian cholesterol and is essential for maintaining the integrity and fluidity of the yeast cell membrane. Inhibition of ERG11 disrupts ergosterol synthesis, leading to impaired membrane function and increased cell permeability. Research areas related to ERG11 include antifungal drug development, as this enzyme is the target of azole antifungal drugs. Understanding the structure and function of ERG11 is crucial for developing new antifungal therapies and combating fungal infections.

Target Background

Function

Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.

Gene References into Functions

molecular characterization of Erg11 including interactions with NADPH-cytochrome P450 reductase and role of Erg11 in antifungal resistance [REVIEW] PMID: 22217856

Subcellular Location

Membrane; Single-pass membrane protein.

Protein Families

Cytochrome P450 family

Database Links

KEGG: sce:YHR007C

STRING: 4932.YHR007C

Code	CSB-EP006459SVG1
Abbreviation	Recombinant Saccharomyces cerevisiae ERG11 protein, partial
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Saccharomyces cerevisiae Lanosterol 14-alpha demethylase (ERG11), partial

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