Recombinant Saccharomyces cerevisiae Lipase 4 (TGL4), partial

Code CSB-YP330548SVG
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP330548SVG
MSDS
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Source E.coli
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Code CSB-EP330548SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP330548SVG
MSDS
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Source Baculovirus
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Code CSB-MP330548SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TGL4
Uniprot No.
Alternative Names
TGL4; STC1; YKR089C; YKR409; Lipase 4; EC 3.1.1.3; Triacylglycerol lipase 4
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Lipid particle-localized triacylglycerol (TAG) lipase. The lipid droplet/particle is a lipid storage compartment which serves as a depot of energy and building blocks for membrane lipid biosynthesis. Involved in the mobilization of the non-polar storage lipids triacylglycerols (TAGs) from lipid particles by hydrolysis of TAGs, releasing and supplying specific fatty acids to the appropriate metabolic pathways. Also has steryl ester (SE) hydrolase and phospholipase A(2) (PLA(2)) activities, and catalyzes the acylation of lysophosphatidic acid (LPA). Contributes to early bud formation in late G1 phase of the cell cycle upon phosphorylation and activation by cyclin-dependent kinase 1 (Cdk1/CDC28).
Gene References into Functions
  1. Multiple functions as lipase, steryl ester hydrolase, phospholipase, and acyltransferase of Tgl4p from the yeast Saccharomyces cerevisiae. PMID: 20332534
  2. A genetically and functionally related network, consisting of components of the Rim101 pathway and the prefoldin, retromer and SWR1 complexes, and predict its functional relation to PLA(2) activity in a model eukaryotic cell. PMID: 20379744
  3. The identification of Tgl4p and Tgl5p as additional TAG lipases of the yeast is reported. PMID: 16135509
  4. yeast Tgl4 is a functional ortholog of mammalian adipose triglyceride lipase PMID: 16267052
Subcellular Location
Lipid droplet.
Database Links

KEGG: sce:YKR089C

STRING: 4932.YKR089C

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