Recombinant Saccharomyces cerevisiae Mediator of RNA polymerase II transcription subunit 10 (NUT2)

Code CSB-YP013641SVG
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Source Yeast
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Code CSB-EP013641SVG
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Source E.coli
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Code CSB-EP013641SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013641SVG
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Source Baculovirus
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Code CSB-MP013641SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NUT2
Uniprot No.
Alternative Names
NUT2; MED10; YPR168W; P9325.2; Mediator of RNA polymerase II transcription subunit 10; Mediator complex subunit 10; Negative regulator of URS2 protein 2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-157
Target Protein Sequence
MNGNSTNNEQ LQQELATTQD QVASIIESFV ELGVSIYDFP GTPEATKGMI TNLQRNVDRL YKLNVRSNDP QSSLSKVDIP LEVVQYIEDG RNPDIYTREF VEAIRRSNQY QRGKMHGLKQ LRDSLADKIV DEFPELKEPV EDIIKRTSPI DNVSNTH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.
Gene References into Functions
  1. impact of Mediator med10 mutation on PIC formation and transcription has amplitude, which is gene-dependent and correlated with the promoter architecture in terms of TATA elements, nucleosome occupancy, and dynamics. PMID: 27688401
Subcellular Location
Nucleus.
Protein Families
Mediator complex subunit 10 family
Database Links

KEGG: sce:YPR168W

STRING: 4932.YPR168W

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