Recombinant Saccharomyces cerevisiae Meiosis-specific serine/threonine-protein kinase MEK1 (MEK1)

Code CSB-YP333015SVG
MSDS
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Source Yeast
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Code CSB-EP333015SVG
MSDS
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Source E.coli
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Code CSB-EP333015SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333015SVG
MSDS
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Source Baculovirus
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Code CSB-MP333015SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MEK1
Uniprot No.
Alternative Names
MEK1; MRE4; YOR351C; O6357; Meiosis-specific serine/threonine-protein kinase MEK1; EC 2.7.11.1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-497
Target Protein Sequence
MRPLYSCNLA TKDDIEMAGG VAPAHLEVNV GGYNTEQTIP IVKHQLVKVG RNDKECQLVL TNPSISSVHC VFWCVFFDED SIPMFYVKDC SLNGTYLNGL LLKRDKTYLL KHCDVIELSQ GSEENDIKKT RLVFMINDDL QSSLDPKLLD QMGFLREVDQ WEITNRIVGN GTFGHVLITH NSKERDEDVC YHPENYAVKI IKLKPNKFDK EARILLRLDH PNIIKVYHTF CDRNNHLYIF QDLIPGGDLF SYLAKGDCLT SMSETESLLI VFQILQALNY LHDQDIVHRD LKLDNILLCT PEPCTRIVLA DFGIAKDLNS NKERMHTVVG TPEYCAPEVG FRANRKAYQS FSRAATLEQR GYDSKCDLWS LGVITHIMLT GISPFYGDGS ERSIIQNAKI GKLNFKLKQW DIVSDNAKSF VKDLLQTDVV KRLNSKQGLK HIWIAKHLSQ LERLYYKKIL CNNEGPKLES INSDWKRKLP KSVIISQAIP KKKKVLE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable protein kinase required for meiotic recombination.
Gene References into Functions
  1. ndt80 transcription of CDC5 results in degradation of Red1, a meiosis-specific protein required for Mek1 activation, thereby abolishing Mek1 activity completely. Elimination of Mek1 kinase activity allows Rad51-mediated repair of any remaining DSBs. In this way, cells do not enter Meiosis I until recombination is complete and all DSBs are repair PMID: 28249986
  2. When Mek1 persists on closely paired (synapsed) homologues, DNA repair is severely delayed, suggesting that Mek1 suppresses any proximal repair template. PMID: 26870961
  3. chromatin remodeling allows Dot1-mediated H3K79-me to exclude Pch2 from the chromosomes, thus driving localization of Hop1 along chromosome axes and enabling Mek1 full activation to trigger downstream responses, such as meiotic arrest PMID: 23382701
  4. propose that Mek1 plays dual roles during meiotic prophase I by phosphorylating targets directly involved in the recombination checkpoint, as well as targets involved in sister chromatid recombination PMID: 20888230
  5. Hop1 phosphorylation in response to double strand breakage triggers dimerization of Mek1. PMID: 16221890

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Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CHEK2 subfamily
Database Links

KEGG: sce:YOR351C

STRING: 4932.YOR351C

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