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Recombinant Saccharomyces cerevisiae Mitochondrial respiratory chain complexes assembly protein AFG3 (AFG3), partial

Recombinant Saccharomyces cerevisiae Mitochondrial respiratory chain complexes assembly protein AFG3 (AFG3), partial

Code	CSB-YP331099SVG1
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP331099SVG1
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP331099SVG1-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP331099SVG1
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP331099SVG1
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

AFG3

Uniprot No.

P39925

Alternative Names

AFG3; YTA10; YER017C; Mitochondrial respiratory chain complexes assembly protein AFG3; ATPase family gene 3 protein; Tat-binding homolog 10

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Acts as a component of the m-AAA protease complex which is a ATP-dependent metalloprotease mediating degradation of non-assembled mitochondrial inner membrane proteins. The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes. Function both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides.

Gene References into Functions

replacement of the Yta10 TM2 domain abolishes membrane dislocation for only a subset of substrates, whereas replacement of the Yta12 TM2 domain impairs membrane dislocation for all tested substrates, suggesting different roles of the TM domains in each m-AAA protease subunit PMID: 29030426
Observations show a role for the mitochondrial protease Afg3 in modulating cytoplasmic mRNA translation and ER stress resistance, and suggest Afg3 modulates longevity in yeast by a mechanism similar to cytoplasmic ribosomal large subunit proteins. PMID: 23167605

Subcellular Location

Mitochondrion inner membrane; Multi-pass membrane protein.

Protein Families

AAA ATPase family; Peptidase M41 family

Database Links

KEGG: sce:YER017C

STRING: 4932.YER017C

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