Product Details

Purity

>85% (SDS-PAGE)

Target Names

MYO2

Uniprot No.

P19524

Alternative Names

MYO2; CDC66; YOR326W; O6167; Myosin-2; Cell division control protein 66; Class V unconventional myosin MYO2; Type V myosin heavy chain MYO2; Myosin V MYO2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Myosin heavy chain that is required for the cell cycle-regulated transport of various organelles and proteins for their segregation. Functions by binding with its tail domain to receptor proteins on organelles and exerting force with its N-terminal motor domain against actin filaments, thereby transporting its cargo along polarized actin cables. Essential for the delivery of secretory vesicles to sites of active growth during bud emergence and cytokinesis. Required for segregation and inheritance of peroxisomes, late Golgi compartments, mitochondria and the vacuole to the daughter cell during cell division. Also required for correct alignment of the spindle during mitosis.

Gene References into Functions

spatial and temporal regulation of myosin V in vivo by a head-to-tail interaction is critical for the normal delivery functions of the motor. PMID: 25940346
These findings reveal a new fast-acting energy conservation strategy halting growth by immobilizing myosin V in a newly described state on selectively stabilized actin cables. PMID: 25308080
myosin-V is activated by binding a secretory vesicle and that myosin-V mutations that compromise vesicle binding render the motor constitutively active PMID: 23079598
Myo2p is not processive on bare actin but highly processive on actin-tropomyosin. Tpm1p supports the most robust processivity. PMID: 22704989
Myo2p interacts with Pex19p PMID: 22486971
It was shown that Mmr1 functions as a mitochondria-specific cargo adaptor early in the cell cycle and that Mmr1 binds Myo2 at the site that binds Vac17. PMID: 22753895
Yeast myosin-II (Myo1p) and myosin-V (Myo2p) accommodate the reduced N-terminal charge density of yeast actin, showing greater activity with yeast over muscle actin PMID: 21757693
Myo2 is an essential and direct mediator of bud-directed mitochondrial movement in yeast. PMID: 21807878
During polarization, cells change from fast to slow cable dynamics through spatio-temporal regulation of Bni1, Bnr1 and Myo2. PMID: 21486946
Results characterize the interaction between Sro7 and the type V myosin Myo2 and show that this interaction is important for two distinct aspects of Sro7 function. PMID: 21248204
retention of mitochondria in the bud was compromised in YPT11 and MYO2 mutants PMID: 15215313
The globular tail of Myo2p contains two subdomains (I and II) that have distinct functions, vacuole-specific and secretory vesicle-specific movement. Subdomain I tightly associates with subdomain II. PMID: 15684027
Myo2p is subject to phosphoregulation involving a PKA-related signaling pathway. PMID: 16467380
Inp2p is the first peroxisomal protein implicated in the vectorial movement of peroxisomes.Inp2p interacts directly with the globular tail of Myo2p. PMID: 16678774
Considering that a poly-IQ sequence is the lever arm of myosin V, data suggest that the arm may harbor a joint, localized within the IQ4 sequence, enabling the elasticity of the neck of myosin V. PMID: 18020453
P-body disassembly is delayed in the motor mutant, myo2-66, indicating that Myo2p may facilitate the release of mRNAs from the P-body. PMID: 18218704
Myo2 plays an important and direct role for mitochondrial motility and inheritance in budding yeast PMID: 18391073
Ypt11 and Ret2 are components of a Myo2 receptor complex that functions during the Golgi inheritance into the growing bud. PMID: 18595704
Fus2p and Sec4p, another Myo2p cargo associated with exocytotic vesicles, reside predominantly on different cellular organelles. PMID: 19403698
Results delineate the region of Myo2p devoted to binding peroxisomes. PMID: 19687257

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Subcellular Location

Bud neck. Bud tip. Note=Concentrates to sites of polarized growth, namely to the bud tip during S and G2 phases of the cell cycle and to the bud neck during cytokinesis.

Protein Families

TRAFAC class myosin-kinesin ATPase superfamily, Myosin family

Database Links

KEGG: sce:YOR326W

STRING: 4932.YOR326W

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Recombinant Saccharomyces cerevisiae Myosin-2 (MYO2), partial

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