Recombinant Saccharomyces cerevisiae Nuclear mRNA export protein THP1 (THP1)

Code CSB-YP600842SVG
MSDS
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Source Yeast
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Code CSB-EP600842SVG
MSDS
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Source E.coli
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Code CSB-EP600842SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP600842SVG
MSDS
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Source Baculovirus
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Code CSB-MP600842SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
THP1
Uniprot No.
Alternative Names
THP1; BUD29; YOL072W; O1140; Nuclear mRNA export protein THP1; Bud site selection protein 29
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-455
Target Protein Sequence
MDMANQLLDE LAHGNFSHLT LNLSQNGREI AILQKQLTGF DDKQLETFVE QHPAMPNDTR FKIMCTSFLN YARDVDPWSA WSSSDLIFEF YQCLINCLIN DNAPHIEMLI PVATRETEFI INLAGKLDSF HLQLHTRSHQ FLSHISSILS RLFNSIKPPR GNASSTNIPG KQRILLYLVN KLNNIYFRIE SPQLCSNIFK NFQPKSMLAH FNEYQLDQQI EYRYLLGRYY LLNSQVHNAF VQFNEAFQSL LNLPLTNQAI TRNGTRILNY MIPTGLILGK MVKWGPLRPF LSQETIDNWS VLYKHVRYGN IQGVSLWLRQ NERHLCARQL LIVLLEKLPM VTYRNLIKTV IKSWTTEWGQ NKLPYSLIER VLQLSIGPTF EDPGAQEITI YNGIHSPKNV ENVLVTLINL GLLRANCFPQ LQLCVVKKTT MIQEIVPPVN ERITKMFPAH SHVLW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the SAC3-THP1 complex, which functions in transcription-coupled mRNA export from the nucleus to the cytoplasm. SAC3-THP1 functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket), by association with components of the nuclear mRNA export machinery (MEX67-MTR2 and SUB2) in the nucleoplasm and the nucleoporin NUP1 at the nuclear basket. THP1 binds to RNA in vitro.
Subcellular Location
Nucleus envelope. Note=Localizes to the nuclear pores.
Database Links

KEGG: sce:YOL072W

STRING: 4932.YOL072W

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