Recombinant Saccharomyces cerevisiae Nucleolar protein 3 (NPL3)

Code CSB-YP015921SVG
MSDS
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Source Yeast
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Code CSB-EP015921SVG
MSDS
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Source E.coli
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Code CSB-EP015921SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015921SVG
MSDS
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Source Baculovirus
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Code CSB-MP015921SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NPL3
Uniprot No.
Alternative Names
NPL3; MTR13; MTS1; NAB1; NOP3; YDR432W; D9461.19; Nucleolar protein 3; Mitochondrial targeting suppressor 1 protein; Nuclear polyadenylated RNA-binding protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-414
Target Protein Sequence
MSEAQETHVE QLPESVVDAP VEEQHQEPPQ APDAPQEPQV PQESAPQESA PQEPPAPQEQ NDVPPPSNAP IYEGEESHSV QDYQEAHQHH QPPEPQPYYP PPPPGEHMHG RPPMHHRQEG ELSNTRLFVR PFPLDVQESE LNEIFGPFGP MKEVKILNGF AFVEFEEAES AAKAIEEVHG KSFANQPLEV VYSKLPAKRY RITMKNLPEG CSWQDLKDLA RENSLETTFS SVNTRDFDGT GALEFPSEEI LVEALERLNN IEFRGSVITV ERDDNPPPIR RSNRGGFRGR GGFRGGFRGG FRGGFSRGGF GGPRGGFGGP RGGYGGYSRG GYGGYSRGGY GGSRGGYDSP RGGYDSPRGG YSRGGYGGPR NDYGPPRGSY GGSRGGYDGP RGDYGPPRDA YRTRDAPRER SPTR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for pre-rRNA processing and nuclear import as well as mitochondrial protein targeting. Binds to poly(A).
Gene References into Functions
  1. Npl3 participates in DNA double-strand break resection by promoting the proper biogenesis of EXO1 mRNA. PMID: 28472517
  2. a model in which Hmt1, via arginine methylation of Npl3, facilitates U1 snRNP engagement with the pre-mRNA to promote usage of non-consensus splice sites by the splicing machinery. PMID: 28392442
  3. Npl3 binds diverse sites on large numbers of transcripts, and the loss of Npl3 results in transcriptional readthrough on many genes. PMID: 26694144
  4. An essential novel function for Npl3 in the cytoplasm, which supports monosome formation for translation initiation. PMID: 24100011
  5. yeast Npl3, the most abundant RNA-binding hnRNP, prevents R-loop-mediated genome instability PMID: 24240235
  6. our data reveal a connection between Npl3 and an extensive array of chromatin factors and describe an unanticipated functional link between histone H2B ubiquitination and pre-mRNA splicing PMID: 23209445
  7. Npl3 and Cbc2 may function in maintaining telomere stability perhaps as telomere capping proteins. PMID: 22902537
  8. These results support a model in which arginine methylation facilitates Npl3 export directly by weakening contacts with nuclear proteins. PMID: 15998636
  9. NPL1p can bind to nascent mRNAs, heat shock RNA was exported efficiently in temperature-sensitive NPl13 mutant strains PMID: 17259545
  10. The results support a model in which balanced competition of Npl3 with mRNA processing factors may promote recognition of proper polyadenylation sites while suppressing cryptic sites. PMID: 17684230
  11. Improved segmental isotope labeling methods for the NMR study of multidomain of of large proteins: application to the RRMs of NPL3. PMID: 17936301
  12. Structure of NPL3 protein and its interaction with mRNA 3'-end processing signals is reported. PMID: 18022637
  13. Proper levels of Npl3 protein are achieved by a negative feedback loop in which phosphorylated Npl3 suppresses efficient recognition of the productive processing signals in its own transcript. PMID: 18391019
  14. Npl3 has a role in RNA polymerase II elongation and its regulation by phosphorylation PMID: 18818768
  15. A single SR-like protein, Npl3, is required for efficient splicing of a large set of pre-mRNAs in Saccharomyces cerevisiae PMID: 19061647
  16. Nuclear localization of Npl3 also requires specific arginine-glycine (RG) sequences, yet heterologous RG domains allow similar modulation of Npl3 transport by arginine methylation. PMID: 19454603
  17. model in which Npl3p engenders translational fidelity by promoting the remodeling of mRNPs during translation termination PMID: 19733178

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Subcellular Location
Nucleus, nucleolus.
Protein Families
RRM GAR family
Database Links

KEGG: sce:YDR432W

STRING: 4932.YDR432W

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