Recombinant Saccharomyces cerevisiae Peflin (PEF1)

Code CSB-YP017771SVG
MSDS
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Source Yeast
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Code CSB-EP017771SVG
MSDS
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Source E.coli
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Code CSB-EP017771SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017771SVG
MSDS
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Source Baculovirus
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Code CSB-MP017771SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PEF1
Uniprot No.
Alternative Names
PEF1; YGR058W; Peflin; Penta-EF hand domain-containing protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-335
Target Protein Sequence
MCAKKLKYAA GDDFVRYATP KEAMEETRRE FEKEKQRQQQ IKVTQAQTPN TRVHSAPIPL QTQYNKNRAE NGHHSYGSPQ SYSPRHTKTP VDPRYNVIAQ KPAGRPIPPA PTHYNNLNTS AQRIASSPPP LIHNQAVPAQ LLKKVAPASF DSREDVRDMQ VATQLFHNHD VKGKNRLTAE ELQNLLQNDD NSHFCISSVD ALINLFGASR FGTVNQAEFI ALYKRVKSWR KVYVDNDING SLTISVSEFH NSLQELGYLI PFEVSEKTFD QYAEFINRNG TGKELKFDKF VEALVWLMRL TKLFRKFDTN QEGIATIQYK DFIDATLYLG RFLPH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Calcium-binding protein that is required for polar bud growth and cell wall abscission. Can also bind zinc ions.
Gene References into Functions
  1. Our results provide further evidence for a linkage between Ca(2+)-dependent signaling and ER-to-Golgi trafficking, but its mechanism of action in yeast seems to be different from the mechanism reported for its mammalian homolog ALG-2. PMID: 22792405
  2. This study reports the identification of a unique PEF protein in Saccharomyces cerevisiae encoded by the uncharacterized open reading frame YGR058w. PMID: 17640275
Subcellular Location
Cytoplasm. Nucleus. Bud tip. Bud neck.
Database Links

KEGG: sce:YGR058W

STRING: 4932.YGR058W

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