Recombinant Saccharomyces cerevisiae Peroxisomal membrane protein PEX14 (PEX14)

Code CSB-YP017800SVG
MSDS
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Source Yeast
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Code CSB-EP017800SVG
MSDS
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Source E.coli
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Code CSB-EP017800SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017800SVG
MSDS
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Source Baculovirus
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Code CSB-MP017800SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PEX14
Uniprot No.
Alternative Names
PEX14; YGL153W; G1870; Peroxisomal membrane protein PEX14; Peroxin-14
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
2-341
Target Protein Sequence
SDVVSKDRK ALFDSAVSFL KDESIKDAPL LKKIEFLKSK GLTEKEIEIA MKEPKKDGIV GDEVSKKIGS TENRASQDMY LYEAMPPTLP HRDWKDYFVM ATATAGLLYG AYEVTRRYVI PNILPEAKSK LEGDKKEIDD QFSKIDTVLN AIEAEQAEFR KKESETLKEL SDTIAELKQA LVQTTRSREK IEDEFRIVKL EVVNMQNTID KFVSDNDGMQ ELNNIQKEME SLKSLMNNRM ESGNAQDNRL FSISPNGIPG IDTIPSASEI LAKMGMQEES DKEKENGSDA NKDDNAVPAW KKAREQTIDS NASIPEWQKN TAANEISVPD WQNGQVEDSI P
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the peroxisomal translocation machinery with PEX13 and PEX17. Interacts with both the PTS1 and PTS2 receptors. Binds directly to PEX17.
Gene References into Functions
  1. Pex14p contains two independent Pex5p binding sites, one in the N- and one in the C-terminus. PMID: 15949802
  2. Pex14p is required in only catalytic amounts to allow initiation of the selective peroxisome degradation process. PMID: 16874074
Subcellular Location
Peroxisome membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Peroxin-14 family
Database Links

KEGG: sce:YGL153W

STRING: 4932.YGL153W

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