Recombinant Saccharomyces cerevisiae Pre-mRNA-splicing factor 18 (PRP18)

Code CSB-YP018760SVG
MSDS
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Source Yeast
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Code CSB-EP018760SVG
MSDS
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Source E.coli
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Code CSB-EP018760SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018760SVG
MSDS
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Source Baculovirus
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Code CSB-MP018760SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PRP18
Uniprot No.
Alternative Names
PRP18; YGR006W; Pre-mRNA-splicing factor 18
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-251
Target Protein Sequence
MDLDLASILK GEISKKKKEL ANSKGVQPPC TEKFQPHESA NIDETPRQVE QESTDEENLS DNQSDDIRTT ISKLENRPER IQEAIAQDKT ISVIIDPSQI GSTEGKPLLS MKCNLYIHEI LSRWKASLEA YHPELFLDTK KALFPLLLQL RRNQLAPDLL ISLATVLYHL QQPKEINLAV QSYMKLSIGN VAWPIGVTSV GIHARSAHSK IQGGRNAANI MIDERTRLWI TSIKRLITFE EWYTSNHDSL A
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the U4/U5/U6 snRNP, binding principally to the u5 snRNP. It is not absolutely required for the second step of pre-mRNA splicing at low temperatures but is required at higher temperatures. It may stabilize a particular conformation of the U5 snRNP or orient the U5 snRNP within the U4/U5/U6 snRNP or within the spliceosome.
Gene References into Functions
  1. structure along with sequence comparisons among SRA1p orthologs and against authentic RNA recognition motif (RRM) proteins indicates that it is not an RRM domain but rather an all-helical protein with a fold more similar to the PRP18 splicing factor PMID: 24486611
  2. Prp18 acts in concert with U5 snRNA during the second step of pre-mRNA splicing. PMID: 15743809
  3. The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicing. PMID: 17504938
  4. Arginine1753 of Prp8 collaborates with Prp18 to stabilize U5 snRNA/exon contacts that are important for the second transesterification step of pre-mRNA splicing. PMID: 17626844
Subcellular Location
Nucleus.
Protein Families
PRP18 family
Database Links

KEGG: sce:YGR006W

STRING: 4932.YGR006W

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