Recombinant Saccharomyces cerevisiae Probable glycosidase CRH1 (CRH1)

Code CSB-YP344875SVG
MSDS
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Source Yeast
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Code CSB-EP344875SVG
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Source E.coli
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Code CSB-EP344875SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP344875SVG
MSDS
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Source Baculovirus
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Code CSB-MP344875SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CRH1
Uniprot No.
Alternative Names
CRH1; YGR189C; G7553Probable glycosidase CRH1; EC 3.2.-.-; Congo red hypersensitive protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
23-482
Target Protein Sequence
ESTATADS TTAASSTASC NPLKTTGCTP DTALATSFSE DFSSSSKWFT DLKHAGEIKY GSDGLSMTLA KRYDNPSLKS NFYIMYGKLE VILKAANGTG IVSSFYLQSD DLDEIDIEWV GGDNTQFQSN FFSKGDTTTY DRGEFHGVDT PTDKFHNYTL DWAMDKTTWY LDGESVRVLS NTSSEGYPQS PMYLMMGIWA GGDPDNAAGT IEWAGGETNY NDAPFTMYIE KVIVTDYSTG KKYTYGDQSG SWESIEADGG SIYGRYDQAQ EDFAVLANGG SISSSSTSSS TVSSSASSTV SSSVSSTVSS SASSTVSSSV SSTVSSSSSV SSSSSTSPSS STATSSKTLA SSSVTTSSSI SSFEKQSSSS SKKTVASSST SESIISSTKT PATVSSTTRS TVAPTTQQSS VSSDSPVQDK GGVATSSNDV TSSTTQISSK YTSTIQSSSS EASSTNSVQI SN
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable glycosidase that plays a role in cell wall architecture. Required for the transfer of chitin to 1,6-beta-glucan in the cell wall.
Gene References into Functions
  1. catalytic properties of Crh1 yeast cell wall transglycosylases PMID: 23919454
  2. Crh1 and Crh2 catalyze the transfer of chitin to both beta(1-3)- and beta(1-6)glucan in cell wall. PMID: 19734368
  3. Study indicates that Crh1p and Crh2p, homologues of glycosyltransferases, ferry chitin chains from chitin synthase III to beta(1-6)glucan. PMID: 17302808
  4. Crh1p and Crh2p act as transglycosylases in vivo and in vitro PMID: 18694928
Subcellular Location
Secreted, cell wall. Membrane; Lipid-anchor, GPI-anchor. Note=Covalently-linked GPI-modified cell wall protein (GPI-CWP), localized particularly in chitin-rich areas. Found at the incipient bud site, around the septum area in later stages of budding, and in ascospore envelopes. Redistributes uniformly over the cell cortex upon heat stress.
Protein Families
Glycosyl hydrolase 16 family, CRH1 subfamily
Database Links

KEGG: sce:YGR189C

STRING: 4932.YGR189C

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