Recombinant Saccharomyces cerevisiae Protein ATS1 (ATS1)

Code CSB-YP330078SVG
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP330078SVG
MSDS
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Source E.coli
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Code CSB-EP330078SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP330078SVG
MSDS
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Source Baculovirus
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Code CSB-MP330078SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ATS1
Uniprot No.
Alternative Names
ATS1; KTI13; YAL020C; FUN28; YAL006Protein KTI13; Alpha-tubulin suppressor 1; Kluyveromyces lactis toxin-insensitive protein 13
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-333
Target Protein Sequence
MSCVYAFGSN GQRQLGLGHD EDMDTPQRSV PGDDGAIVRK IACGGNHSVM LTNDGNLVGC GDNRRGELDS AQALRQVHDW RPVEVPAPVV DVACGWDTTV IVDADGRVWQ RGGGCYEFTQ QHVPLNSNDE RIAVYGCFQN FVVVQGTRVY GWGSNTKCQL QEPKSRSLKE PVLVYDTGSV AVDYVAMGKD FMVIVDEGGR IVHASGRLPT GFELKQQQKR HNLVVLCMWT SIHLWNARLN TVESFGRGTH SQLFPQERLD FPIVGVATGS EHGILTTANQ EGKSHCYNVY CWGWGEHGNC GPQKGSQPGL QLVGQYSGKP RVFGGCATTW IVL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Together with KTI11; associates with the elongator and is required for tRNA Wobble base modifications mediated by the elongator complex. Association with the elongator complex is mediated via interaction with KTI11. The elongator complex is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s 2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine). Together with KTI11; also required for diphthamide biosynthesis; diphthamide is a post-translational modification of histidine which occurs in elongation factor 2. May participate in regulatory interactions between microtubules and the cell cycle.
Gene References into Functions
  1. Insight into the molecular mechanisms that allow the Kti11/Kti13 heterodimer to coregulate two consecutive steps in ribosomal protein synthesis. PMID: 25543256
  2. Kti11 forms a stable 1 : 1 complex with Kti13.In the complex, Kti13 orients Kti11 and restricts access to its electron-carrying iron atom, constraining the electron transfer capacity of Kti11. PMID: 25604895
  3. Kti13 and Kti11 support Elongator functions and they both share Elongator-independent roles that are important for cell viability. PMID: 18466297
Subcellular Location
Cytoplasm.
Database Links

KEGG: sce:YAL020C

STRING: 4932.YAL020C

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