Recombinant Saccharomyces cerevisiae Protein BTN2 (BTN2)

Code CSB-YP344873SVG
MSDS
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Source Yeast
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Code CSB-EP344873SVG
MSDS
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Source E.coli
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Code CSB-EP344873SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP344873SVG
MSDS
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Source Baculovirus
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Code CSB-MP344873SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BTN2
Uniprot No.
Alternative Names
BTN2; YGR142WProtein BTN2; Batten disease protein 2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-410
Target Protein Sequence
MFSIFNSPCV FEQLPSFSQP LHSRYFDCSS PVSYYPECKR RKAIKANLRA PKKSDANCSE PLRYALAETP NGYTLSLSKR IPYELFSKYV NEKLGELKEN HYRPTYHVVQ DFFGNQYYVE DEADEDALLR SALKDLDFRA IGKKIAKDLF QDYEIELNHR GDELSILSKK DKIFKEFSLD QVFEDVFVIG CGVENIDDGS REKYALLKIG LVKHEEEISE GGINEPKMPI IESKIDESHD DVNMSESLKE EEAEKAKEPL TKEDQIKKWI EEERLMQEES RKSEQEKAAK EDEERQKKEK EARLKARKES LINKQKTKRS QQKKLQNSKS LPISEIEASN KNNNSNSGSA ESDNESINSD SDTTLDFSVS GNTLKKHASP LLEDVEDEEV DRYNESLSRS PKGNSIIEEI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
V-SNARE binding protein that facilitates specific protein retrieval from a late endosome to the Golgi. Modulates the rate of arginine uptake. Involved in pH homeostasis. Required for the correct localization of IST2. May be involved in ion homeostasis together with IST2.
Gene References into Functions
  1. Juxtanuclear deposits of misfolded protein reside in an intranuclear quality control compartment whose formation is dependent on Btn2. PMID: 25672362
  2. Normal levels of the antiprion proteins Btn2 and Cur1 cure most newly formed [URE3] prion variants. PMID: 24938787
  3. Protein redistribution by Btn2 is accompanied by a gradual depletion of Sis1 from the cytosol, which is mediated by the sorting factor Cur1. PMID: 22718905
  4. Btn2-mediated endosomal protein trafficking and prion curing in yeast is regulated by Btn3 PMID: 21441304
  5. Btn2 facilitates specific protein retrieval from a late endosome to the Golgi apparatus, a process which may be adversely affected in patients with Batten disease. PMID: 17101785
  6. Btn2p is part of a system that collects aggregates preventing their efficient distribution to progeny cells PMID: 18833194

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Subcellular Location
Cytoplasm. Late endosome.
Database Links

KEGG: sce:YGR142W

STRING: 4932.YGR142W

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