Recombinant Saccharomyces cerevisiae Protein IFH1(IFH1) ,partial

Code CSB-YP340112SVG
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Source Yeast
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Code CSB-EP340112SVG
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Source E.coli
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Code CSB-EP340112SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP340112SVG
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Source Baculovirus
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Code CSB-MP340112SVG
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names IFH1
Uniprot No. P39520
Alternative Names IFH1; RRP3; YLR223C; L8083.9; Protein IFH1; Ribosomal RNA-processing protein 3
Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Controls the pre-rRNA processing machinery in conjunction with FHL1. Could convert FHL1 from a repressor to an activator.
Gene References into Functions
  1. The role of CK2-dependent phosphorylation of Ifh1 and Crf1 in transcriptional regulation of ribosomal protein genes in Saccharomyces cerevisiae has been described. PMID: 27321754
  2. A dynamic Ifh1-Utp22 interaction fine-tunes ribosomal protein gene (RPG) expression to coordinate RPG and rRNA transcription. PMID: 27818142
  3. data suggest a novel mechanism of regulation whereby Gcn5 functions to titrate the activity of Ifh1 following its recruitment to RP promoters to provide more than an all-or-nothing mode of transcriptional regulation PMID: 23973296
  4. activation domain (AD) of the ribosomal protein genes activator Ifh1p was identified. PMID: 20059977
  5. Ifh1 association with promoters is the key regulatory step for coordinate expression of ribosomal protein genes PMID: 15616568
  6. central role for Ifh1 and Fhl1 in ribosomal protein gene regulation PMID: 15616569
  7. Fhl1p and Ifh1p are key players in the transcription of ribosomal protein genes PMID: 15692568
  8. the interaction between Fhl1p and Ifh1p is critical for the regulation of CPC2 transcription during utilization of different carbon sources. PMID: 16402205

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Subcellular Location Nucleus.
Protein Families IFH1 family
Database Links

KEGG: sce:YLR223C

STRING: 4932.YLR223C

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