Recombinant Saccharomyces cerevisiae Protein KES1 (KES1)

Code CSB-YP336093SVG
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP336093SVG
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP336093SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP336093SVG
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP336093SVG
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
KES1
Uniprot No.
Alternative Names
KES1; OSH4; YPL145C; LPI3C; P2614Oxysterol-binding protein homolog 4; Protein KES1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-434
Target Protein Sequence
MSQYASSSSW TSFLKSIASF NGDLSSLSAP PFILSPISLT EFSQYWAEHP ELFLEPSFIN DDNYKEHCLI DPEVESPELA RMLAVTKWFI STLKSQYCSR NESLGSEKKP LNPFLGELFV GKWENKEHPE FGETVLLSEQ VSHHPPVTAF SIFNDKNKVK LQGYNQIKAS FTKSLMLTVK QFGHTMLDIK DESYLVTPPP LHIEGILVAS PFVELEGKSY IQSSTGLLCV IEFSGRGYFS GKKNSFKARI YKDSKDSKDK EKALYTISGQ WSGSSKIIKA NKKEESRLFY DAARIPAEHL NVKPLEEQHP LESRKAWYDV AGAIKLGDFN LIAKTKTELE ETQRELRKEE EAKGISWQRR WFKDFDYSVT PEEGALVPEK DDTFLKLASA LNLSTKNAPS GTLVGDKEDR KEDLSSIHWR FQRELWDEEK EIVL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol with phosphatidylinositol 4-phosphate (PI4P), delivering sterol to the Golgi in exchange for PI4P, which is degraded by the SAC1 phosphatase in the endoplasmic reticulum. Displays a similar affinity for PI4P and sterols. Binds sterol and PI4P in a mutually exclusive manner (Probable).
Gene References into Functions
  1. the sterol content in the mitochondrial fraction was significantly decreased in vivo after Osh4 inactivation in a genetic background in which all the other OSH genes were deleted. PMID: 29487131
  2. Osh4-mediated exocytosis depends upon lipid binding and exchange without a strict requirement for sterol. PMID: 28993464
  3. Osh4p transports sterol against its gradient between two membranes by dissipating the energy of a phosphatidylinositol-4-phosphate gradient. PMID: 25849868
  4. Osh4p is needed to reduce the level of phosphatidylinositol-4-phosphate on secretory vesicles as they mature. PMID: 25165144
  5. X-ray crystallographic determination of the conformational changes Osh4 undergoes during ligand binding. PMID: 23756172
  6. Kes1 (Osh4p) associated with exocytic vesicles that move from the mother cell into the bud. PMID: 21819498
  7. Study reports that Kes1 integrates multiple aspects of lipid metabolism in distal stages of the secretory pathway with TORC1 and nitrogen signaling. PMID: 22341443
  8. The structure of the Osh4p-phosphatidylinositol 4-phosphate complex was solved. It was shown that Osh4p quickly exchanges dehydroergosterol for phosphatidylinositol 4-phosphate. PMID: 22162133
  9. structure of the open state of a lid-truncated form of Osh4 was determined at 2.5 A resolution PMID: 16136145
  10. Osh4p/Kes1p, specifically facilitates the nonvesicular transfer of cholesterol and ergosterol between the plasma membrane and endoplasmic reticulum in vitro PMID: 16585271
  11. Kes1p regulates Golgi apparatus phosphatidylinositol-4-phosphate function PMID: 17881569
  12. The mechanism used by Osh4 to release cholesterol is conceptualized as a molecular ladder with the rungs being amino acids or water-mediated amino acids that interact with 3-hydroxyl group of cholesterol PMID: 18937371

Show More

Hide All

Subcellular Location
Golgi apparatus membrane.
Protein Families
OSBP family
Database Links

KEGG: sce:YPL145C

STRING: 4932.YPL145C

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1