Recombinant Saccharomyces cerevisiae Protein PRY1 (PRY1)

Code CSB-YP344027SVG
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP344027SVG
MSDS
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Source E.coli
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Code CSB-EP344027SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP344027SVG
MSDS
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Source Baculovirus
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Code CSB-MP344027SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PRY1
Uniprot No.
Alternative Names
PRY1; YJL079C; J1022; Protein PRY1; Pathogenesis-related protein 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
20-299
Target Protein Sequence
A PAVVTVTEHA HEAAVVTVQG VVYVENGQTR TTYETLAPAS TATPTSTATA LVAPPVAPSS ASSNSDVVLS ALKNLASVWG KTTDSTTTLT SSESTSQSLA QATTTSTPAA ASTTSTPAAT TTTSQAAATS SASSSDSDLS DFASSVLAEH NKKRALHKDT PALSWSDTLA SYAQDYADNY DCSGTLTHSG GPYGENLALG YDGPAAVDAW YNEISNYDFS NPGFSSNTGH FTQVVWKSTT QVGCGIKTCG GAWGDYVICS YDPAGNYEGE YADNVEPLA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Secreted protein required for efficient export of lipids such as acetylated sterols. Acts in detoxification of hydrophobic compounds.
Gene References into Functions
  1. These results indicate that some CAP family members, such as Pry1, can bind different lipids, particularly sterols and fatty acids, at distinct binding sites, suggesting that the CAP domain may serve as a stable, secreted protein domain that can accommodate multiple ligand-binding sites. PMID: 28365570
  2. the caveolin-binding motif of Pry1 is crucial for selective lipid binding and that lipid binding may occur through displacement of the loop containing this motif PMID: 24598142
Subcellular Location
Secreted.
Protein Families
CRISP family
Database Links

KEGG: sce:YJL079C

STRING: 4932.YJL079C

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