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Recombinant Saccharomyces cerevisiae Protein RIF2 (RIF2)

Recombinant Saccharomyces cerevisiae Protein RIF2 (RIF2)

Code	CSB-YP312340SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP312340SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP312340SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP312340SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP312340SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

RIF2

Uniprot No.

Q06208

Alternative Names

RIF2; YLR453C; Protein RIF2; RAP1-interacting factor 2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Expression Region

1-395

Target Protein Sequence

MEHVDSDFAP IRRSKKVVDS DKIVKAISDD LEQKNFTVLR KLNLVPIKKS VSSPKVCKPS PVKERVDHVF YQKFKSMALQ ELGTNYLSIS YVPSLSKFLS KNLRSMKNCI VFFDKVEHIH QYAGIDRAVS ETLSLVDINV VIIEMNDYLM KEGIQSSKSK ECIESMGQAS YSGQLDFEAS EKPSNHTSDL MMMVMRKINN DESIDHIVYF KFEQLDKLST STIIEPSKLT EFINVLSVLE KSNNIAFKVL IYSNNVSISS LLSTSLKKKL NTKYTVFEMP ILTCAQEQEY LKKMIKFTFD SGSKLLQSYN SLVTCQLNNK ESNLAIFFEF LKVFPHPFTY LFNAYTEIIV QSRTFDELLD KIRNRLTIKN YPHSAYNFKK NQRLPLKLTR KVHDR

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Involved in transcriptional silencing and telomere length regulation. Its role in telomere length regulation results from either a block in elongation or promoting degradation of the telomere ends. Loss of RIF1 function results in derepression of an HMR silencer, whose ARS consensus element has been deleted, and in the elongation of telomeres. RAP1 may target the binding of RIF1 to silencers and telomeres.

Gene References into Functions

We identified an N-terminal domain in Rif2 that is essential for length regulation, which we have termed BAT domain for Blocks Addition of Telomeres PMID: 26294668
The role of Tel1 in promoting MRX accumulation to double-strand breaks (DSBs)is counteracted by Rif2, which is recruited to DSBs. We also found that Rif2 enhances ATP hydrolysis by MRX and attenuates MRX function in end-tethering. PMID: 26901759
The X-ray structures of Rif1 and Rif2 bound to the Rap1 C-terminal domain and that of the Rif1 C terminus are presented. Both Rif1 and Rif2 have separable and independent Rap1-binding epitopes, allowing Rap1 binding over large distances (42-110 A). PMID: 23746845
The telomeric-bound protein, Rif2, was also found to promote a telomere fold-back through the recruitment of Rpd3L to telomeres PMID: 23028367
Rif2 is unable to negatively regulate processing and lengthening at TEL1-hy909 telomeres, indicating that the Tel1-hy909 variant overcomes the inhibitory activity exerted by Rif2 on Mre11-Rad50-Xrs2 PMID: 22354991
Data show that DNA ends that are protected by Yku and Rif2 involves multiple functions of the MRX complex that do not necessarily require its nuclease activity. PMID: 21152442
Data show that the Rap1 C-terminal domain establishes two parallel inhibitory pathways through the proteins Rif2 and Sir4. PMID: 18451106