Product Details

Purity

>85% (SDS-PAGE)

Target Names

RPN4

Uniprot No.

Q03465

Alternative Names

RPN4; SON1; UFD5; YDL020C; D2840; Protein RPN4; Nuclear protein SON1; Ubiquitin fusion degradation protein 5

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Acts as a transcriptional activator of a number of genes encoding proteasomal subunits. Binds to a PACE (proteasome-associated control element) DNA sequence 5'-GGTGGCAAA-3'. Its expression is in turn regulated by the 26S proteasome, thereby providing a negative feedback control mechanism. Required for normal growth at low temperatures.

Gene References into Functions

Rpn4 induces TMC1 transcription in response to misfolded proteins. However, this response is counteracted by rapid proteasome-dependent degradation of Tmc1, which serves to normalize Tmc1 protein levels after induction PMID: 27226598
Rpn4-responsive elements in genes related to proteasome functions PMID: 25747386
under the methyl methanesulfonate stress Rpn4 promotes the regulation of several genes involved in DNA repair, antioxidant response and glucose metabolism. PMID: 25842837
the ubiquitin-independent degradation of Rpn4 requires the 19S regulatory particle. PMID: 22349505
evidence for the role of proteasomal degradation of Rpn4 in nonhomologous end-joining PMID: 20376190
Intracellular proteasome activity is correlated with the transactivation potency of Rpn4. PMID: 19914394
Rpn4 thus represents an important stress-responsive mediator whose degradation as well as availability are critical for cell survival under stressed conditions. PMID: 19933873
analysis of Rpn4 ubiquitination site and ubiquitin-dependent degradation signal PMID: 16492666
expression regulated by HSF in response to heat shock and MMS treatments PMID: 16556235
These results indicate that ubiquitin-mediated degradation of Rpn4 is controlled by a phosphorylation-dependent ubiquitylation signal. PMID: 17532487
These data suggest that Mub1 and Ubr2 cooperate to transfer ubiquitin to Rpn4 from Rad6 and that Mub1 may switch from a partner to a substrate of the Ubr2/Rad6 ubiquitin ligase. PMID: 18070918
This activation of FLR1 transcription is fully dependent on Yap1p and is reduced (by 50%) in the absence of Rpn4p, Yrr1p or Pdr3p. PMID: 18086556
Rpn4p may act as an activator or repressor for the genes of the ubiquitin-proteasome system. PMID: 18702311
Rpn4p devoid of its C-terminal region containing the zinc finger DNA-binding domains and its N-terminus, which shares no homology with transactivation domains of known transcription factors, was completely unable to activate transcription. PMID: 18702312
The Rpn4p transactivation domains were mapped by deletion analysis. PMID: 18804109
the physiological function of Rpn4-induced proteasome expression PMID: 18832351
The requirement for Rpn4p reflects severe impairment of the proteasome by unfolded protein response-membrane stress. PMID: 19073890

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Subcellular Location

Nucleus.

Database Links

KEGG: sce:YDL020C

STRING: 4932.YDL020C

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Recombinant Saccharomyces cerevisiae Protein RPN4 (RPN4), partial

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