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Recombinant Saccharomyces cerevisiae Protein SPT10 (SPT10), partial

Recombinant Saccharomyces cerevisiae Protein SPT10 (SPT10), partial

Code	CSB-YP333981SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP333981SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP333981SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP333981SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP333981SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

SPT10

Uniprot No.

P35208

Alternative Names

SPT10; CRE1; SUD1; YJL127C; J0702; Protein SPT10

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Required for normal transcription at a number of loci in yeast. Affects transcription at Ty1 elements, at PHO5, STE6 and ADH2.

Gene References into Functions

Spt10 is critical for normal cell division. PMID: 23450643
suggest an interaction between Grx5 and SPT10 via intermolecular disulfide linkage and propose a model for a role of Grx5 in the regulation of protein expression under the control of SPT10 PMID: 22326886
Spt10 and Spt21 control silencing in S. cerevisiae by altering chromatin structure through roles beyond the control of histone gene expression. PMID: 21057056
mutations lethal when combined with spt21 mutations PMID: 15744051
SPT10, a putative acetyltransferase, is required for cell cycle-specific K56 acetylation at histone genes. PMID: 15882620
Spt10p is a sequence-specific activator of the histone genes, possessing a DNA-binding domain fused to a likely histone acetyltransferase (HAT) domain. PMID: 16199888
the C388S mutation disables the DNA-binding function of Spt10p in vitro and in vivo PMID: 16415340
Spt10p is a dimer and that cooperative binding to the histone upstream activating sequences correlates with dimer formation, which is mediated through its N-terminal domain. PMID: 17202156