Recombinant Saccharomyces cerevisiae Protein UPS2, mitochondrial (UPS2)

Code CSB-YP330437SVG
MSDS
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Source Yeast
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Code CSB-EP330437SVG
MSDS
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Source E.coli
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Code CSB-EP330437SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP330437SVG
MSDS
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Source Baculovirus
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Code CSB-MP330437SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
UPS2
Uniprot No.
Alternative Names
UPS2; AIM30; GEP1; MSF1; YLR168C; L9470.15; Protein UPS2; mitochondrial; Altered inheritance rate of mitochondrion protein 30; Genetic interactor of prohibitins protein 1; Unprocessed MGM1 protein 2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-230
Target Protein Sequence
MKLFQNSYDF NYPWDQVTAA NWKKYPNEIS THVIAVDVLR RELKDQGKVL VTERLITVKQ GVPKWIMMML GGTNMSHVRE VSVVDLNKKS LTMRSCNLTM CNLLKVYETV TYSPHPDDSA NKTLFQQEAQ ITAYGSIRKL CNKMEDWSVQ RFCENAKKGK MGFDAVLQVF SENWEKHVDD LSNQLVSKVN ETMEDVKISA GTLLKGTERS GRTILQQNID LFRDAYNHEN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for mitochondrial cristae morphogenesis and MGM1-processing. Controls the stability of mitochondrial phosphatidylethanolamine (PE). With UPS1, controls the level of cardiolipin in mitochondria. Cardiolipin is a unique phospholipid with four fatty acid chains and is present mainly in the mitochondrial inner membrane where it stabilizes the electron transport chain supercomplex between complexes III and IV through direct interaction of their subunits.
Gene References into Functions
  1. PS is transferred from the mitochondrial outer membrane to the inner membrane independently of Psd1p, Ups1p, and Ups2p and decarboxylated to PE by Psd1p in the inner membrane PMID: 23124206
  2. Data suggest that Ups1p and Up2p proteins may function in phospholipid trafficking between the outer and inner mitochondrial membranes. PMID: 22403410
  3. The authors show that Ups1, Ups2 and Ups3 protein import is mediated by another mitochondrial intermembrane space protein, Mdm35p. PMID: 20622808
  4. The authors identified Mdm35, a member of the twin Cx9C protein family, as a novel interaction partner of Ups1 and Ups2. PMID: 20657548
  5. Data report that two homologous intermembrane space proteins, Ups1p and Ups2p, control cardiolipin metabolism and affect the assembly state of TIM23 and its association with PAM in an opposing manner. PMID: 19506038

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Subcellular Location
Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side. Mitochondrion intermembrane space. Note=Lacks the two major intermembrane space-targeting signals, bipartite presequences and cysteine motifs, and import is mediated by another IMS protein, MDM35.
Protein Families
Slowmo family
Database Links

KEGG: sce:YLR168C

STRING: 4932.YLR168C

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