Recombinant Saccharomyces cerevisiae Protein VHS2 (VHS2)

Code CSB-YP328118SVG
MSDS
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Source Yeast
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Code CSB-EP328118SVG
MSDS
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Source E.coli
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Code CSB-EP328118SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP328118SVG
MSDS
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Source Baculovirus
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Code CSB-MP328118SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
VHS2
Uniprot No.
Alternative Names
VHS2; YIL135C; Protein VHS2; Viable in a HAL3 SIT4 background protein 2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-436
Target Protein Sequence
MDTSNHNQDH DSHVAAQREN DNNYMPPSPS MSESSMIFER NVEDPSYLYK TVSNNAANSL SRQSSRTSLF NHNNSSNRNF HNLSQRSSAV NLHLQPSRTN ESIASYQTYN PDFVVQTPLD HRRTLENFVP PALDAGCSIV TDDTTGLDDV DMVYSRRPST IGLDRALGRT RSLSSQSFDN ETSPAHPRSP NDHGSRLLRF YSYADMLSDD NNNNVSNATS TSSTANPLRR PPMQGHYSFS SSLLNSPSHL PSPPSASASP PQHMNFTNPF IISRRYSNTT INNANGGTSA GSTTGAALSR SPSNQQYLLK QQRSPSGSAR SRRNSNRPGS AANIMIGKPK SKFHMESSGS EGFSSEEEDN TMIERDKLNL KQKLQSQLAQ PPSIANMVND NHNNTNKHKN TINNNIKNSP AFTNSNPSSK SNSNSTITSM NPDTTK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Can suppress the synthetic lethality of the hal3 sit4 double mutation when overexpressed, suggesting that it is involved in the G1-S transition.
Gene References into Functions
  1. Cyclin-dependent protein kinase Cdk1 and protein phosphatase Cdc14 control Vhs2 post-translational modifications. Vhs2 is a novel Cdc14 substrate that is involved in the control of septin organization. PMID: 24646733
  2. VHS2 regulates polarization of the actin cytoskeleton and cell growth and function in a pathway distinct from and parallel to GIC1 and GIC2. PMID: 16816427
Subcellular Location
Cytoplasm.
Database Links

KEGG: sce:YIL135C

STRING: 4932.YIL135C

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