Recombinant Saccharomyces cerevisiae Recombination protein 107 (REC107)

Code CSB-YP324234SVG
MSDS
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Source Yeast
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Code CSB-EP324234SVG
MSDS
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Source E.coli
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Code CSB-EP324234SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP324234SVG
MSDS
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Source Baculovirus
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Code CSB-MP324234SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
REC107
Uniprot No.
Alternative Names
REC107; MER2; YJR021C; J1462; Recombination protein 107; Meiotic recombination protein 2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-314
Target Protein Sequence
MVARGRTDEI STDVSEANSE HSLMITETSS PFRSIFSHSG KVANAGALEE SDKQILEWAG KLELESMELR ENSDKLIKVL NENSKTLCKS LNKFNQLLEQ DAATNGNVKT LIKDLASQIE NQLDKVSTAM LSKGDEKKTK SDSSYRQVLV EEISRYNSKI TRHVTNKQHE TEKSMRCTQE MLFNVGSQLE DVHKVLLSLS KDMHSLQTRQ TALEMAFREK ADHAYDRPDV SLNGTTLLHD MDEAHDKQRK KSVPPPRMMV TRSMKRRRSS SPTLSTSQNH NSEDNDDASH RLKRAARTII PWEELRPDTL ESEL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Essential for meiotic chromosome segregation. MER1 and MER2 proteins must interact directly or indirectly. MER1 might be responsible for regulating the MER2 gene and/or gene product. MER2 is not required for mitosis and mitotic DNA repair mechanisms. Component of the MER2-MEI4-REC114 complex which seems to be required for meiotic double-strand break (DSB) formation.
Gene References into Functions
  1. Mer2, Mei4, and Rec114 form a distinct complex required for double-strand breaks formation. PMID: 16783010
Subcellular Location
Nucleus. Chromosome. Note=Localizes to chromosomes.
Database Links

KEGG: sce:YJR021C

STRING: 4932.YJR021C

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