Recombinant Saccharomyces cerevisiae Replication factor A protein 2 (RFA2)

Code CSB-YP329660SVG
MSDS
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Source Yeast
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Code CSB-EP329660SVG
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Source E.coli
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Code CSB-EP329660SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP329660SVG
MSDS
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Source Baculovirus
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Code CSB-MP329660SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RFA2
Uniprot No.
Alternative Names
RFA2; BUF1; YNL312W; N0368; Replication factor A protein 2; RF-A protein 2; DNA-binding protein BUF1; Replication protein A 36 kDa subunit
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-273
Target Protein Sequence
MATYQPYNEY SSVTGGGFEN SESRPGSGES ETNTRVNTLT PVTIKQILES KQDIQDGPFV SHNQELHHVC FVGVVRNITD HTANIFLTIE DGTGQIEVRK WSEDANDLAA GNDDSSGKGY GSQVAQQFEI GGYVKVFGAL KEFGGKKNIQ YAVIKPIDSF NEVLTHHLEV IKCHSIASGM MKQPLESASN NNGQSLFVKD DNDTSSGSSP LQRILEFCKK QCEGKDANSF AVPIPLISQS LNLDETTVRN CCTTLTDQGF IYPTFDDNNF FAL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.
Gene References into Functions
  1. Post-translational modification of the Rfa2 N-terminus is not required for cells to deal with DNA damage; however, it might influence whether cells proceed into M-phase in the continued presence of unrepaired DNA lesions. PMID: 25595672
  2. Results suggests that RPA2 is necessary for structural integrity and may also be needed for intra- and/or inter-protein interactions. PMID: 19244309
Subcellular Location
Nucleus.
Protein Families
Replication factor A protein 2 family
Database Links

KEGG: sce:YNL312W

STRING: 4932.YNL312W

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