Recombinant Saccharomyces cerevisiae Ribosomal RNA-processing protein 8 (RRP8)

Code CSB-YP020531SVG
MSDS
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Source Yeast
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Code CSB-EP020531SVG
MSDS
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Source E.coli
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Code CSB-EP020531SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP020531SVG
MSDS
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Source Baculovirus
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Code CSB-MP020531SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RRP8
Uniprot No.
Alternative Names
RRP8; YDR083W; D4461; 25S rRNA; adenine(645)-N(1))-methyltransferase; EC 2.1.1.287; Ribosomal RNA-processing protein 8
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-392
Target Protein Sequence
MALFNVEGWS IKTKTVAFDN KTNKSSKDKK KNNRKNGKLT REQKLKEETE AELKEQVEDI PSEGSVAKDI PKKNQEKSDQ NETSKKRKHD EEAPLMQVKE NIEKPTKKQL TPLQQKMMAK LTGSRFRWIN EQLYTISSDE ALKLIKEQPQ LFDEYHDGFR SQVQAWPENP VDVFVDQIRY RCMKPVNAPG GLPGLKDSKE IVIADMGCGE AQLALEINNF FKNYNKKAKK YLKRRHKVHS FDLKKANERI TVADIRNVPL PDESCTIVVF CLALMGTNFL DFIKEAYRIL APRGELWIAE IKSRFSDGKG NEFVDALKLM GFFHKKTFDE NKMFTRFEFF KPPAEIIEER RQKLERRQKF IEVETEKEEL EKKRRKIAEG KWLLKPCIYK RR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of adenine 645 in 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis. Required for efficient pre-rRNA cleavage at site A2. Also involved in telomere length regulation and maintenance.
Gene References into Functions
  1. The data highlight a dual functionality of Rrp8 in the biogenesis of both small and large ribosomal subunits. PMID: 23180764
Subcellular Location
Nucleus, nucleolus. Chromosome, telomere.
Protein Families
Methyltransferase superfamily, RRP8 family
Database Links

KEGG: sce:YDR083W

STRING: 4932.YDR083W

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