Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase RIM11/MSD1 (RIM11)

Code CSB-YP327862SVG
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP327862SVG
MSDS
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Source E.coli
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Code CSB-EP327862SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP327862SVG
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP327862SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RIM11
Uniprot No.
Alternative Names
RIM11; GSK3; MDS1; YMR139W; YM9375.08; Serine/threonine-protein kinase RIM11/MSD1; EC 2.7.11.1; Regulator of IME2 protein 11
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-370
Target Protein Sequence
MNIQSNNSPN LSNNIVSKQV YYAHPPPTID PNDPVQISFP TTEVVGHGSF GVVFATVIQE TNEKVAIKKV LQDKRFKNRE LEIMKMLSHI NIIDLKYFFY ERDSQDEIYL NLILEYMPQS LYQRLRHFVH QRTPMSRLEI KYYMFQLFKS LNYLHHFANV CHRDIKPQNL LVDPETWSLK LCDFGSAKQL KPTEPNVSYI CSRYYRAPEL IFGATNYTNQ IDIWSSGCVM AELLLGQPMF PGESGIDQLV EIIKILGTPS KQEICSMNPN YMEHKFPQIK PIPLSRVFKK EDDQTVEFLA DVLKYDPLER FNALQCLCSP YFDELKLDDG KINQITTDLK LLEFDENVEL GHLSPDELSS VKKKLYPKSK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Serine/threonine protein kinase that is thought to function in regulating kinetochore activity and entry into meiosis. Could phosphorylate IME1.
Gene References into Functions
  1. Yeast GSK-3 kinase regulates astral microtubule function through phosphorylation of the microtubule-stabilizing kinesin Kip2 PMID: 26395399
  2. GSK-3 is required for optimal myo-inositol-3 phosphate synthase activity and de novo inositol biosynthesis PMID: 17257308
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, GSK-3 subfamily
Database Links

KEGG: sce:YMR139W

STRING: 4932.YMR139W

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