Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase RIO1 (RIO1)

Code CSB-YP613205SVG
MSDS
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Source Yeast
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Code CSB-EP613205SVG
MSDS
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Source E.coli
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Code CSB-EP613205SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP613205SVG
MSDS
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Source Baculovirus
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Code CSB-MP613205SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RIO1
Uniprot No.
Alternative Names
RIO1; RRP10; YOR119C; O3266; YOR3266C; Serine/threonine-protein kinase RIO1; EC 2.7.11.1; EC 3.6.3.-; Ribosomal RNA-processing protein 10
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-484
Target Protein Sequence
MSLEDKFDSL SVSQGASDHI NNQLLEKYSH KIKTDELSFS RAKTSKDKAN RATVENVLDP RTMRFLKSMV TRGVIADLNG CLSTGKEANV YHAFAGTGKA PVIDEETGQY EVLETDGSRA EYAIKIYKTS ILVFKDRERY VDGEFRFRNS RSQHNPRKMI KIWAEKEFRN LKRIYQSGVI PAPKPIEVKN NVLVMEFLSR GNGFASPKLK DYPYKNRDEI FHYYHTMVAY MRLLYQVCRL VHADLSEYNT IVHDDKLYMI DVSQSVEPEH PMSLDFLRMD IKNVNLYFEK MGISIFPERV IFQFVISETL EKFKGDYNNI SALVAYIASN LPIKSTEQDE AEDEIFRSLH LVRSLGGLEE RDFDRYTDGK FDLLKSLIAH DNERNFAASE QFEFDNADHE CSSGTEEFSD DEEDGSSGSE EDDEEEGEYY DDDEPKVLKG KKHEDKDLKK LRKQEAKDAK REKRKTKVKK HIKKKLVKKT KSKK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for the final endonucleolytic cleavage at site D converting 20S pre-rRNA into the mature 18S rRNA. Required for the final steps of cytoplasmic maturation of the 40S ribosomal subunit. The association with the very late 40S subunit intermediate seems to follow RIO2 association with precursors of the 40S subunit and may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit. Despite the protein kinase domain is proposed to act predominantly as an ATPase. The catalytic activity regulates its dynamic association with the 40S subunit. Has a role in the cell cycle where it is required for entrance into S-phase and in the control of the onset of anaphase. Appears to also be involved in the maintenance of chromosome stability and correct mitotic segregation.
Gene References into Functions
  1. The study indicates that lack of Rio1p allows premature entry of pre-40S particles in the translation process. PMID: 28977579
  2. Nuclear Rio1 promotes rDNA array stability and segregation. PMID: 25851096
Subcellular Location
Cytoplasm.
Protein Families
Protein kinase superfamily, RIO-type Ser/Thr kinase family
Database Links

KEGG: sce:YOR119C

STRING: 4932.YOR119C

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