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Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase STE20 (STE20), partial

Recombinant Saccharomyces cerevisiae Serine/threonine-protein kinase STE20 (STE20), partial

Code	CSB-YP311785SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP311785SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP311785SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP311785SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP311785SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

STE20

Uniprot No.

Q03497

Alternative Names

STE20; YHL007C; Serine/threonine-protein kinase STE20; EC 2.7.11.1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

MAP4K component of the MAPK pathway required for the mating pheromone response, haploid invasive growth and diploid pseudohyphal development. Links the pheromone response G-protein beta gamma subunits to downstream signaling components. Needed for mating in haploid cells, induction of a mating-specific gene FUS1, induction of mating-specific morphologies, and pheromone-induced proliferation arrest. Required for the regulation of the actin polarization and bud emergence during cell cycle in G1. Involved in the high osmolarity glycerol (HOG) response. Phosphorylates 'Thr-307' and 'Ser-302' or 'Ser-306' of STE11 and 'Ser-357' of MYO3. Phosphorylates histone H2B to form H2BS10ph during meiosis and H(2)O(2)-induced apoptosis. Its interaction with CDC42 is required for both invasive growth and the formation of pseudohyphae. Its interaction with STE4 is required for the pheromone signaling.

Gene References into Functions

during filamentous growth, Ste20 stimulates V-ATPase activity, this would sustain nutrient mobilization from vacuolar stores, which is beneficial for filamentous growth. PMID: 22327006
cell cycle kinases Ste20 as central mediators of the cellular response to inhibition of fatty acid synthesis. (Ste20) PMID: 22218487
An in silico predictor was built to identify Ste20p substrates on a proteome-wide basis. PMID: 20020052
An evolutionarily conserved binding site for Bem1 between the CRIB and kinase domains of Ste20 was identified. PMID: 15743816
In mitogen-activated protein kinase (MAPK) cascades of budding yeast, pheromone-induced mating signal is transmitted by interactions between the beta-subunit of a G-protein (G-beta) and the G-beta binding (GBB) domain of Ste20 kinase. PMID: 16870141
to gain further insight into the mechanisms of Ste20 action in cell polarity, we screened for interactors of Ste20; among the identified proteins were Erg4, Cbr1 and Ncp1, which are all involved in sterol biosynthesis PMID: 17895367

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Subcellular Location

Cytoplasm. Nucleus. Note=The translocation from the cytoplasm to the nucleus is stimulated by H(2)O(2). Localizes at bud emergence during cell cycle and the shmoo top during mating, both localizations requiring an interaction with CDC42.

Protein Families

Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily

Database Links

KEGG: sce:YHL007C

STRING: 4932.YHL007C

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