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Recombinant Saccharomyces cerevisiae Signaling mucin MSB2 (MSB2), partial

Recombinant Saccharomyces cerevisiae Signaling mucin MSB2 (MSB2), partial

Code	CSB-YP330156SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP330156SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP330156SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP330156SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP330156SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

MSB2

Uniprot No.

P32334

Alternative Names

MSB2; YGR014W; Signaling mucin MSB2; Multicopy suppressor of bud emergence 2; Osmosensor MSB2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Plasma membrane signaling mucin that promotes activation of the MAPK for the filamentous growth pathway. Partially redundant with the SHO1 osmosensing branch for the activation of STE11.

Gene References into Functions

Msb2 is required for pressure sensing. Msb2 is linked to the actin cortex. PMID: 29187529
The extracellular cysteine-rich domain of the transmembrane-anchor protein Opy2 binds to the Hkr1-Msb2 homology domain of the putative osmosensor Msb2 and the formation of the Opy2-Msb2 complex is essential for osmotic activation of Hog1. PMID: 26598606

Subcellular Location

Cell membrane; Single-pass membrane protein. Note=localized to polarized sites on the cell surface.

Protein Families

HKR1/MSB2 family

Database Links

KEGG: sce:YGR014W

STRING: 4932.YGR014W

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