Recombinant Saccharomyces cerevisiae Sorting nexin-3 (SNX3)

Code CSB-YP600902SVG
MSDS
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Source Yeast
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Code CSB-EP600902SVG
MSDS
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Source E.coli
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Code CSB-EP600902SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP600902SVG
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP600902SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SNX3
Uniprot No.
Alternative Names
SNX3; GRD19; YOR357C; Sorting nexin-3; Golgi retention deficient protein 19
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-162
Target Protein Sequence
MPREFKSFGS TEKSLLSKGH GEPSYSEIYA EPENFLEIEV HNPKTHIPNG MDSKGMFTDY EIICRTNLPS FHKRVSKVRR RYSDFEFFRK CLIKEISMLN HPKVMVPHLP GKILLSNRFS NEVIEERRQG LNTWMQSVAG HPLLQSGSKV LVRFIEAEKF VG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for retention of late Golgi membrane proteins. Component of the retrieval machinery that functions by direct interaction with the cytosolic tails of certain TGN membrane proteins during the sorting/budding process at the prevacuolar compartment. Binds phosphatidylinositol 3-phosphate (PtdIns(P3)).
Gene References into Functions
  1. Grd19/Snx3p functions as a cargo-specific adapter for the retromer complex, establishing a precedent for a mechanism by which sorting nexins expand the repertoire of retromer-dependent cargos. PMID: 17420293
  2. In the presence and absence of iron, Fet3-Ftr1 transits an endosomal compartment where a subunit of the multivesicular body sorting receptor (Vps27), Snx3/Grd19, and retromer proteins colocalize. PMID: 18768754
Subcellular Location
Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Prevacuolar compartment membrane; Peripheral membrane protein; Cytoplasmic side. Note=Mainly cytoplasmic. A minor amount associates with membranes.
Protein Families
Sorting nexin family
Database Links

KEGG: sce:YOR357C

STRING: 4932.YOR357C

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