Recombinant Saccharomyces cerevisiae Sphingolipid long chain base-responsive protein PIL1 (PIL1)

Code CSB-YP346841SVG
MSDS
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Source Yeast
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Code CSB-EP346841SVG
MSDS
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Source E.coli
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Code CSB-EP346841SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP346841SVG
MSDS
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Source Baculovirus
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Code CSB-MP346841SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PIL1
Uniprot No.
Alternative Names
PIL1; YGR086C; Sphingolipid long chain base-responsive protein PIL1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-339
Target Protein Sequence
MHRTYSLRNS RAPTASQLQN PPPPPSTTKG RFFGKGGLAY SFRRSAAGAF GPELSRKLSQ LVKIEKNVLR SMELTANERR DAAKQLSIWG LENDDDVSDI TDKLGVLIYE VSELDDQFID RYDQYRLTLK SIRDIEGSVQ PSRDRKDKIT DKIAYLKYKD PQSPKIEVLE QELVRAEAES LVAEAQLSNI TRSKLRAAFN YQFDSIIEHS EKIALIAGYG KALLELLDDS PVTPGETRPA YDGYEASKQI IIDAESALNE WTLDSAQVKP TLSFKQDYED FEPEEGEEEE EEDGQGRWSE DEQEDGQIEE PEQEEEGAVE EHEQVGHQQS ESLPQQTTA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Negative regulator of cell wall integrity (CWI) in unstressed cells, probably by inhibiting protein kinase PKH1/PHK2 activity and regulating their downstream CWI pathways PKC1-MAP kinase pathway and protein kinase YPK1 pathway. Activity may be regulated by the transient increase of sphingolipid long chain bases (LCBs) during heat stress.
Gene References into Functions
  1. Seg1/Sle1 controls eisosome assembly and shape, which also involves Pil1 and Lsp1 PMID: 22869600
  2. Pil1 and Lsp1 self-assemble into higher-order structures and bind preferentially to phosphoinositide-containing membranes. PMID: 22123866
  3. Pil1, an eisosome organizer, plays an important role in the recruitment of synaptojanins and amphiphysins to facilitate receptor-mediated endocytosis in yeast PMID: 21872358
  4. Loss of Pil1 leads to clustering of eisosome remnants and redirects endocytosis and endocytic effector proteins to these clusters PMID: 16496001
  5. Pil1 expression is regulated by the cell cycle, which synchronizes eisosome formation with plasma membrane growth. PMID: 19037108

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Subcellular Location
Lipid droplet.
Database Links

KEGG: sce:YGR086C

STRING: 4932.YGR086C

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