Recombinant Saccharomyces cerevisiae Sporulation-specific mitogen-activated protein kinase SMK1 (SMK1)

Code CSB-YP340437SVG
MSDS
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Source Yeast
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Code CSB-EP340437SVG
MSDS
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Source E.coli
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Code CSB-EP340437SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP340437SVG
MSDS
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Source Baculovirus
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Code CSB-MP340437SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SMK1
Uniprot No.
Alternative Names
SMK1; YPR054W; YP9499.10; Sporulation-specific mitogen-activated protein kinase SMK1; MAP kinase SMK1; EC 2.7.11.24
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-388
Target Protein Sequence
MNCTLTDNTR AINVASNLGA PQQRTIFAKE RISIPGYYEI IQFLGKGAYG TVCSVKFKGR SPAARIAVKK ISNIFNKEIL LKRAIRELKF MNFFKGHKNI VNLIDLEIVT SSPYDGLYCY QELIDYDLAK VIHSSVQLSE FHIKYFLYQI LCGLKYIHSA DVIHRDLKPG NILCTLNGCL KICDFGLARG IHAGFFKCHS TVQPHITNYV ATRWYRAPEL LLSNQPYSKS VDIWAVGCIL AEFYARKPVF MGRDSMHQIF EIIKVLGTPD KDILIKFGTI KAWNLGKNSN NPVYKKIPWS NIFPFASHEA INLIESLLHW DSTHRLNVEQ AISHPFLNEV RKPDDEPVCL QGPFDFTYES ELNSMSKLRD YLVEEVKNFK TDLSSSSL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for spore wall assembly. Required for proper deposition of the two outer layers of the spore wall, the chitosan and dityrosine layers. Negatively regulates GSC2, an alternate catalytic subunit of the 1,3-beta-glucan synthase (GS). Participates in a developmentally regulated signal transduction pathway that coordinates cytodifferentiation events with the transcriptional program.
Gene References into Functions
  1. The T residue in Smk1's activation motif is phosphorylated by the cyclin-dependent kinase (CDK)-activating kinase, Cak1. PMID: 23207907
Protein Families
Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
Database Links

KEGG: sce:YPR054W

STRING: 4932.YPR054W

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