Recombinant Saccharomyces cerevisiae Sterol 24-C-methyltransferase (ERG6)

Code CSB-YP326516SVG
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP326516SVG
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP326516SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP326516SVG
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP326516SVG
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
ERG6
Uniprot No.
Alternative Names
ERG6; ISE1; LIS1; SED6; YML008C; YM9571.10CSterol 24-C-methyltransferase; EC 2.1.1.41; Delta(24)-sterol C-methyltransferase
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
2-383
Target Protein Sequence
SETELRKRQ AQFTRELHGD DIGKKTGLSA LMSKNNSAQK EAVQKYLRNW DGRTDKDAEE RRLEDYNEAT HSYYNVVTDF YEYGWGSSFH FSRFYKGESF AASIARHEHY LAYKAGIQRG DLVLDVGCGV GGPAREIARF TGCNVIGLNN NDYQIAKAKY YAKKYNLSDQ MDFVKGDFMK MDFEENTFDK VYAIEATCHA PKLEGVYSEI YKVLKPGGTF AVYEWVMTDK YDENNPEHRK IAYEIELGDG IPKMFHVDVA RKALKNCGFE VLVSEDLADN DDEIPWYYPL TGEWKYVQNL ANLATFFRTS YLGRQFTTAM VTVMEKLGLA PEGSKEVTAA LENAAVGLVA GGKSKLFTPM MLFVARKPEN AETPSQTSQE ATQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol.
Gene References into Functions
  1. Lis1 has two opposing modes of regulating dynein, being capable of inducing both low and high affinity for the microtubule; study shows that these opposing modes depend on the stoichiometry of Lis1 binding to dynein and that this stoichiometry is regulated by the nucleotide state of dynein's AAA3 domain. PMID: 28886386
  2. Data show that the erg6Delta mutant strain is more sensitive to hyperosmotic shocks than is WT. PMID: 21081111
  3. Erg28p may not only anchor the C-4 demethylation enzyme complex to the ER but also acts as a protein bridge to the Erg6p enzyme required for the next ergosterol biosynthetic step. PMID: 15522820
  4. photolabeling & mutagenesis were performed on 24-C-sterol methyltransferase to elucidate location & role of active site residues involved in AdoMet binding & catalysis; results indicate that one or both of Cys128 & Pro133 are covalently bound to AdoMet PMID: 18563465
  5. Overexpression of ERG6 and ERG2 led to an increased ergosterol level. PMID: 19835168

Show More

Hide All

Protein Families
Class I-like SAM-binding methyltransferase superfamily, Erg6/SMT family
Database Links

KEGG: sce:YML008C

STRING: 4932.YML008C

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1