Recombinant Saccharomyces cerevisiae Thiamine thiazole synthase (THI4)

Code CSB-YP335715SVG
MSDS
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Source Yeast
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Code CSB-EP335715SVG
MSDS
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Source E.coli
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Code CSB-EP335715SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP335715SVG
MSDS
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Source Baculovirus
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Code CSB-MP335715SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
THI4
Uniprot No.
Alternative Names
THI4; ESP35; MOL1; YGR144W; G6620; Thiamine thiazole synthase; EC 2.4.2.60; Thiazole biosynthetic enzyme
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-326
Target Protein Sequence
MSATSTATST SASQLHLNST PVTHCLSDIV KKEDWSDFKF APIRESTVSR AMTSRYFKDL DKFAVSDVII VGAGSSGLSA AYVIAKNRPD LKVCIIESSV APGGGSWLGG QLFSAMVMRK PAHLFLQELE IPYEDEGDYV VVKHAALFIS TVLSKVLQLP NVKLFNATCV EDLVTRPPTE KGEVTVAGVV TNWTLVTQAH GTQCCMDPNV IELAGYKNDG TRDLSQKHGV ILSTTGHDGP FGAFCAKRIV DIDQNQKLGG MKGLDMNHAE HDVVIHSGAY AGVDNMYFAG MEVAELDGLN RMGPTFGAMA LSGVHAAEQI LKHFAA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.
Gene References into Functions
  1. structure of thiazole synthase (Thi4) from Saccharomyces cerevisiae was determined to 1.8 A resolution PMID: 16964967
  2. observations confirm NAD as the substrate for THI4 and elucidate the early steps of this unique biosynthesis of the thiazole moiety of thiamin in eukaryotes PMID: 17309261
Subcellular Location
Cytoplasm. Nucleus. Note=Excluded from the vacuole.
Protein Families
THI4 family
Database Links

KEGG: sce:YGR144W

STRING: 4932.YGR144W

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