Recombinant Saccharomyces cerevisiae Trans-aconitate 3-methyltransferase (TMT1)

Code CSB-YP409744STA
MSDS
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Source Yeast
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Code CSB-EP409744STA
MSDS
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Source E.coli
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Code CSB-EP409744STA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP409744STA
MSDS
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Source Baculovirus
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Code CSB-MP409744STA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TMT1
Uniprot No.
Alternative Names
TMT1; TAM1; SCY_1678; Trans-aconitate 3-methyltransferase; EC 2.1.1.145
Species
Saccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Expression Region
2-299
Target Protein Sequence
STFSASDFN SERYSSSRPS YPSDFYKMID EYHDGERKLL VDVGCGPGTA TLQMAQELKP FEQIIGSDLS ATMIKTAEVI KEGSPDTYKN VSFKISSSDD FKFLGADSVD KQKIDMITAV ECAHWFDFEK FQRSAYANLR KDGTIAIWGY ADPIFPDYPE FDDLMIEVPY GKQGLGPYWE QPGRSRLRNM LKDSHLDPEL FHDIQVSYFC AEDVRDKVKL HQHTKKPLLI RKQVTLMEFA DYVRTWSAYH QWKQDPKNKD KEDVADWFIK ESLRRRPELS TNTKIEVVWN TFYKLGKRV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate and 3-isopropylmalate at high affinity and of other molecules like cis-aconitate, isocitrate, and citrate at lower velocities and affinities. The function of trans-aconitate methylation appears to be in reducing the toxicity of this spontaneous breakdown product of cis-aconitate. The role of 3-isopropylmalate methylation is unclear but may represent a metabolic branch at 3-isopropylmalate, where some of the material is taken in the pathway leading to leucine and some is taken in a pathway to the 3-isopropylmalate methyl ester, a molecule that provides a signal to switch from vegetative to invasive growth in response to amino acid starvation.
Subcellular Location
Cytoplasm.
Protein Families
Methyltransferase superfamily, Tam family
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