Recombinant Saccharomyces cerevisiae Transcriptional modulator WTM1 (WTM1)

Code CSB-YP622467SVG
MSDS
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Source Yeast
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Code CSB-EP622467SVG
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Source E.coli
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Code CSB-EP622467SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP622467SVG
MSDS
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Source Baculovirus
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Code CSB-MP622467SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WTM1
Uniprot No.
Alternative Names
WTM1; YOR230W; YOR50-20Transcriptional modulator WTM1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-437
Target Protein Sequence
MPKKVWKSST PSTYEHISSL RPKFVSRVDN VLHQRKSLTF SNVVVPDKKN NTLTSSVIYS QGSDIYEIDF AVPLQEAASE PVKDYGDAFE GIENTSLSPK FVYQGETVSK MAYLDKTGET TLLSMSKNGS LAWFKEGIKV PIHIVQELMG PATSYASIHS LTRPGDLPEK DFSLAISDFG ISNDTETIVK SQSNGDEEDS ILKIIDNAGK PGEILRTVHV PGTTVTHTVR FFDNHIFASC SDDNILRFWD TRTSDKPIWV LGEPKNGKLT SFDCSQVSNN LFVTGFSTGI IKLWDARAAE AATTDLTYRQ NGEDPIQNEI ANFYHAGGDS VVDVQFSATS SSEFFTVGGT GNIYHWNTDY SLSKYNPDDT IAPPQDATEE SQTKSLRFLH KGGSRRSPKQ IGRRNTAAWH PVIENLVGTV DDDSLVSIYK PYTEESE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional modulator with roles in meiotic regulation and silencing. Acts either as an adapter to facilitate nuclear import by KAP122 of the RNR2-RNR4 herodimer, also called beta-beta' subunit, which corresponds to the small subunit of the ribonucleotide reductase (RNR); or as an anchor to retain RNR2-RNR4 in the nucleus.
Gene References into Functions
  1. Thus, Wtm1 acts as a nuclear anchor to maintain nuclear localization of Rnr2/4 complexes outside of S phase. In the presence of DNA damage this association is disrupted and Rnr2/Rnr4 become cytoplasmic, where they join with Rnr1 to form an intact complex. PMID: 16452505
  2. WTM1 amplifies transcriptional induction of RNR3 in response to replication stress and modulates expression of genes encoding other RNR subunits. PMID: 16980392
Subcellular Location
Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm.
Database Links

KEGG: sce:YOR230W

STRING: 4932.YOR230W

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