Product Details

Purity

>85% (SDS-PAGE)

Target Names

OPI1

Uniprot No.

P21957

Alternative Names

OPI1; YHL020C; Transcriptional repressor OPI1; Negative regulator of phospholipid biosynthesis; Overproducer of inositol protein 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Expression Region

1-404

Target Protein Sequence

MSENQRLGLS EEEVEAAEVL GVLKQSCRQK SQPSEDVSQA DKMPASESST TPLNILDRVS NKIISNVVTF YDEINTNKRP LKSIGRLLDD DDDEHDDYDY NDDEFFTNKR QKLSRAIAKG KDNLKEYKLN MSIESKKRLV TCLHLLKLAN KQLSDKISCL QDLVEKEQVH PLHKQDGNAR TTTGAGEDET SSDEDDDDEE FFDASEQVNA SEQSIVVKME VVGTVKKVYS LISKFTANSL PEPARSQVRE SLLNLPTNWF DSVHSTSLPH HASFHYANCE EQKVEQQQQQ QQQQQQQQLL QQQLLQQQQQ KRNKDGDDSA SPSSSVTANG KVLILAKESL EMVRNVMGVV DSTLGKAEEW VKQKQEVKEM IRERFLQQQQ QYRQQQQKDG NYVKPSQDNV DSKD

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Negative regulator of the transcriptional complex INO2-INO4 in response to phospholipid precursor availability. When precursors become limiting, OPI1 is retained at the endoplasmic reticulum (ER) and INO2-INO4 activates INO1 and other genes required for phospholipid biosynthesis, whereas abundant precursor availability results in targeting of OPI1 to the nucleus to repress transcription of these genes. Binds directly to phosphatidic acid, which is required for ER targeting and may act as sensing mechanism for precursor availability, as phosphatidic acid becomes rapidly depleted upon phospholipid biosynthesis.

Gene References into Functions

The work shows that transcriptional repressor Opi1p is redox-regulated and establishes a new mechanism of gene regulation involving Opi1p, which is important for adaptation to H2 O2 in yeast cells (Saccharomyces cerevisiae). PMID: 28581036
This study demonstrated that Pho85 binds to Opi1 in vitro and in vivo and that this interaction is increased in the presence of high concentrations of phosphate. PMID: 27743455
Opi1p dissociates from Scs2p after targeting to the nuclear membrane, making it possible to be released from the membrane quickly when phosphatidic acid levels decrease. PMID: 26590299
genes of phospholipid biosynthesis are negatively regulated by repressor protein Opi1 when precursor molecules inositol and choline are available. Opi1-triggered gene repression is mediated by recruitment of the Sin3 corepressor complex. PMID: 22543816
demonstrate that a physical association between the Yet complex and Scs2p-Opi1p is required for proper localization of the Opi1p repressor to endoplasmic reticulum membranes and subsequent INO1 derepression PMID: 21372176
a rapid decrease in intracellular pH in response to glucose starvation regulated binding of phosphatidic acid to Opi1, that coordinately repressed phospholipid metabolic genes; this enabled coupling of membrane biogenesis to nutrient availability PMID: 20798321
Two of the mutations in OPI1 (V343Q and S339F) provide genetic evidence for an interaction between Opi1p and the Ino2p activator since they reside in a region of Opi1p recently shown to interact with Ino2p in vitro. PMID: 16322993
casein kinase II phosphorylation at Ser10 plays a role in stimulating the repression of INO1 when Opi1p was not in its most active state PMID: 16407309
To better understand the mechanism of Opi1p repression, the viable yeast deletion set was screened to identify Opi(-) mutants. In total, 89 Opi(-) mutants were identified, of which 7 were previously known to have the Opi(-) phenotype. PMID: 16582425
This study revealed that a transcription factor that controls INO1 expression, called Opi1p, participates in the regulation of mat formation. Opi1p appears to modulate mat formation by influencing the expression of FLO11. PMID: 16896211

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Subcellular Location

Endoplasmic reticulum. Nucleus. Note=Maintained at the endoplasmic reticulum by SCS2 (PubMed:12727870). In response to elevated inositol levels, translocates to the nucleus (PubMed:15192221).

Database Links

KEGG: sce:YHL020C

STRING: 4932.YHL020C

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Recombinant Saccharomyces cerevisiae Transcriptional repressor OPI1 (OPI1)

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