Recombinant Saccharomyces cerevisiae Trimethylguanosine synthase (TGS1)

Code CSB-YP609119SVG
MSDS
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Source Yeast
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Code CSB-EP609119SVG
MSDS
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Source E.coli
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Code CSB-EP609119SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP609119SVG
MSDS
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Source Baculovirus
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Code CSB-MP609119SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TGS1
Uniprot No.
Alternative Names
TGS1; YPL157W; P2573; Trimethylguanosine synthase; EC 2.1.1.-; Cap-specific guanine-N2 methyltransferase; snRNA/snoRNA cap hypermethylase
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-315
Target Protein Sequence
MGRTFIHASK IKHAARKRKH HSNFRTLIKL LNNDAYKIES SKPLKNGKLF KYWKNRRRLF SKIDSASIYM TDELWFSVTP ERIACFLANF VKACMPNAER ILDVFCGGGG NTIQFAMQFP YVYGVDYSIE HIYCTAKNAQ SYGVDDRIWL KRGSWKKLVS KQKLSKIKYD CVFGSPPWGG PEYLRNDVYD LEQHLKPMGI TKMLKSFLKL SPNVIMFLPR NSDLNQLSRA TRKVLGPFAK CKVLYVKENG YMKGIFCMWG ECFFNYEPAS TENSRRESSE KEELSSENEE LSKRKKHEST TTTKDNTVDI YDVNG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the two serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylates the m3G cap on TLC1 telomerase which affects telomere silencing and telomere length regulation. Required for pre-mRNA splicing, pre-rRNA processing and small ribosomal subunit synthesis. Involved in nucleolar structural organization.
Gene References into Functions
  1. present a biochemical characterization of the human Tgs1 guanine-N2 methyltransferase reaction and identify individual amino acids required for methyltransferase activity in vitro and in vivo PMID: 18775984
  2. methyltransferase Tgs1 has a role in the coordination between telomerase and DNA polymerase for end replication PMID: 18840651
Subcellular Location
Nucleus, nucleolus.
Protein Families
Methyltransferase superfamily, Trimethylguanosine synthase family
Database Links

KEGG: sce:YPL157W

STRING: 4932.YPL157W

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