Recombinant Saccharomyces cerevisiae Tubulin gamma chain (TUB4)

Code CSB-YP347410SVG
MSDS
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Source Yeast
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Code CSB-EP347410SVG
MSDS
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Source E.coli
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Code CSB-EP347410SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP347410SVG
MSDS
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Source Baculovirus
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Code CSB-MP347410SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TUB4
Uniprot No.
Alternative Names
TUB4; YLR212C; L8167.21; Tubulin gamma chain; Gamma-tubulin
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-473
Target Protein Sequence
MGGEIITLQA GQCGNHVGKF LWSQLAKEHA IGTDGLSQLP DSSTERDDDT KPFFRENSRN KFTPRAIMMD SEPSVIADVE NTFRGFFDPR NTWVASDGAS AGNSWANGYD IGTRNQDDIL NKIDKEIDST DNFEGFQLLH SVAGGTGSGL GSNLLEALCD RYPKKILTTY SVFPARSSEV VVQSYNTILA LRRLIEDSDA TVVFDNASLL NISGKVFRNP NIDLQHTNQL ISTIISSVTN SIRFPSYMYS SMSSIYSTLI PSPELHFLSP SFTPFTSDYI HDDIAHKGHS SYDVMLDLLD PSNSLVSTAM NNPTYFNVYN TIIGNVEPRQ ISRAMTKLQQ RIKFPSWSSS AMHVNIGRRS PYLPLQPNEN EVSGMMLSNM STVVNVFENA CNTFDKVFAK GAFLNNYNVG DLFQSMQNVQ DEFAESREVV QSLMEDYVAA EQDSYLDDVL VDDENMVGEL EEDLDADGDH KLV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly. TUB4 is an important spindle pole body component that organizes both cytoplasmic and nuclear microtubule arrays.
Gene References into Functions
  1. findings revealed that Rok1 protein synthesis is repressed by Psk2 and Skp1; this repression appeared to be mediated through the ROK1 uORF1; Tub4 plays a positive role in regulating Rok1 protein synthesis and likely after the uORF1-mediated inhibitory regulation PMID: 26310304
  2. We propose that SPBs, via the tail of Tub4, promote the assembly of functional +TIP complexes before their deployment to microtubule +ends. PMID: 16899509
  3. The structure of the Saccharomyces cerevisiae gamma-tubulin small complex at 25-A resolution by electron microscopy, is determined. PMID: 17978090
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole body.
Protein Families
Tubulin family
Database Links

KEGG: sce:YLR212C

STRING: 4932.YLR212C

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