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Recombinant Saccharomyces cerevisiae UPF0202 protein KRE33 (KRE33), partial

Recombinant Saccharomyces cerevisiae UPF0202 protein KRE33 (KRE33), partial

Code	CSB-YP345333SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP345333SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP345333SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP345333SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP345333SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

KRE33

Uniprot No.

P53914

Alternative Names

KRE33; NAT10; RRA1; YNL132W; N1216; N1858; RNA cytidine acetyltransferase; EC 2.3.1.-; 18S rRNA cytosine acetyltransferase; Killer toxin-resistance protein 33; Ribosomal RNA cytidine acetyltransferase 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

RNA cytidine acetyltransferase with specificity toward both 18S rRNA and tRNAs. Catalyzes the formation of N(4)-acetylcytidine (ac4C) at positions 1280 and 1773 in 18S rRNA. Required for early nucleolar cleavages of precursor rRNA at sites A0, A1 and A2 during 18S rRNA synthesis. Catalyzes the formation of ac4C at position 12 in serine and leucine tRNAs. Requires the tRNA-binding adapter protein TAN1 for full tRNA acetyltransferase activity but not for 18S rRNA acetylation.

Gene References into Functions

Identification of Kre33 as responsible for both 18S rRNA and leucine/serine tRNA acetylation. PMID: 25653167
KRE3 (Rra1p) modulates biogenesis of the 40 S subunit by sensing nuclear acetyl-CoA concentration. PMID: 25086048

Subcellular Location

Nucleus, nucleolus.

Protein Families

RNA cytidine acetyltransferase family, NAT10 subfamily

Database Links

KEGG: sce:YNL132W

STRING: 4932.YNL132W

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