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Recombinant Saccharomyces cerevisiae Ubiquitin carboxyl-terminal hydrolase 10 (UBP10), partial

Recombinant Saccharomyces cerevisiae Ubiquitin carboxyl-terminal hydrolase 10 (UBP10), partial

Code	CSB-YP025696SVG
Size	Pls inquire
Source	Yeast
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Code	CSB-EP025696SVG
Size	Pls inquire
Source	E.coli
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Code	CSB-EP025696SVG-B
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP025696SVG
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP025696SVG
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

UBP10

Uniprot No.

P53874

Alternative Names

UBP10; DOT4; YNL186W; N1619; Ubiquitin carboxyl-terminal hydrolase 10; EC 3.4.19.12; Deubiquitinating enzyme 10; Disrupter of telomere silencing protein 4; Ubiquitin thioesterase 10; Ubiquitin-specific-processing protease 10

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Deubiquitinating enzyme involved in telomere and HM loci silencing, which is the repression of chromatin structure which leads to a stop in the transcription of nearby genes. Targets histone H2B for deubiquitination, thus helping to localize SIR2 to the telomere. At silent chromatin, including telomeres and the rDNA locus, not only maintains low H2B 'Lys-123' ubiquitination (H2BK123Ub), but also low H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). Controls the proliferating-cell nuclear antigen PCNA/POL30 deubiquitination which is crucial for keeping TLS polymerases in check as well as for down-regulating the error-free bypass. Deubiquitinates and stabilizes RPA190, the largest subunit of RNA polymerase I, to achieve optimal levels of ribosomes and cell growth. Protects also nutrient transporters such as GAP1 from ubiquitin-dependent endocytosis.

Gene References into Functions

show that Ubp10 possesses multiple, distinct binding modules within its IDRs that are necessary and sufficient for directing protein interactions important for Ubp10's known roles in gene silencing and ribosome biogenesis PMID: 26149687
In this work we present clear evidence indicating that Ubp10 controls PCNA deubiquitylation in S. cerevisiae PMID: 22829782

Subcellular Location

Nucleus. Chromosome, telomere. Nucleus, nucleolus.

Protein Families

Peptidase C19 family

Database Links

KEGG: sce:YNL186W

STRING: 4932.YNL186W

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