Recombinant Saccharomyces cerevisiae Uncharacterized protein YNL024C (YNL024C)

Code CSB-YP345341SVG
MSDS
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Source Yeast
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Code CSB-EP345341SVG
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Source E.coli
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Code CSB-EP345341SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP345341SVG
MSDS
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Source Baculovirus
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Code CSB-MP345341SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EFM6
Uniprot No.
Alternative Names
EFM6; YNL024C; N2809Protein-lysine N-methyltransferase EFM6; EC 2.1.1.-; Elongation factor methyltransferase 6
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-246
Target Protein Sequence
MESIFGGFGD LVVPRPKEHL GQTDLSFGGK LLPALKICED GGESGCGGKV WIAGELLCEY ILEKSVDHLL SKTVNGTKQF KKVLELGSGT GLVGLCVGLL EKNTFHDGTK VYVTDIDKLI PLLKRNIELD EVQYEVLARE LWWGEPLSAD FSPQEGAMQA NNVDLVLAAD CVYLEEAFPL LEKTLLDLTH CINPPVILMA YKKRRKADKH FFNKIKRNFD VLEITDFSKF EHYLKERTHL FQLIRK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that methylates elongation factor 1-alpha (TEF1 and TEF2) at 'Lys-390'.
Gene References into Functions
  1. We identified most known oxygen-regulated genes and also uncovered new regulated genes. Reverse transcription-quantitative PCR (RT-qPCR) analysis confirmed that the lysine methyltransferase EFM6 and the recombinase DMC1, both conserved in humans, are indeed oxygen-responsive. PMID: 27883312
  2. Ynl024c is the enzyme responsible for methylation of eEF1A at Lys390, and in accordance with prior naming of similar enzymes, we suggest that Ynl024c is renamed to Efm6 (Elongation factor MTase 6). PMID: 26115316
Subcellular Location
Cytoplasm.
Protein Families
Class I-like SAM-binding methyltransferase superfamily, METTL21 family, EFM6 subfamily
Database Links

KEGG: sce:YNL024C

STRING: 4932.YNL024C

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