Recombinant Saccharomyces cerevisiae Vacuolar protein-sorting-associated protein 46 (DID2)

Code CSB-YP304030SVG
MSDS
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Source Yeast
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Code CSB-EP304030SVG
MSDS
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Source E.coli
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Code CSB-EP304030SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP304030SVG
MSDS
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Source Baculovirus
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Code CSB-MP304030SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DID2
Uniprot No.
Alternative Names
DID2; CHM1; FTI1; VPS46; YKR035W-AVacuolar protein-sorting-associated protein 46; Charged multivesicular body protein 1; DOA4-independent degradation protein 2; Fifty two inhibitor 1
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-204
Target Protein Sequence
MSRNSAAGLE NTLFQLKFTS KQLQKQANKA SKEEKQETNK LKRALNENED ISRIYASNAI RKKNERLQLL KLASRVDSVA SRVQTAVTMR QVSASMGQVC KGMDKALQNM NLQQITMIMD KFEQQFEDLD TSVNVYEDMG VNSDAMLVDN DKVDELMSKV ADENGMELKQ SAKLDNVPEI KAKEVNVDDE KEDKLAQRLR ALRG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Class E VPS protein implicated in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. The lumenal sequestrated membrane proteins will be targeted into the vacuole after fusion of the endosome with the vacuole. Probably acts as a peripherally associated component of the ESCRT-III complex, which appears to be critical for late steps in MVB sorting, such as membrane invagination and final cargo sorting and recruits late-acting components of the sorting machinery. The MVB pathway requires the sequential function of ESCRT-O, -I,-II and -III complex assemblies. Regulates the membrane association of VPS4. Can stimulate VPS4 ATPase activity directly or via VTA1.
Gene References into Functions
  1. the ESCRT regulator Did2 coordinates precise maturation of endosomes and thus provides the correct membrane identity for efficient endosomal long-distance transport. PMID: 28422978
  2. Cocrystallization of Ist1NTD with a Did2 fragment shows that Ist1 interacts with the Did2 C-terminal MIM1 (MIT-interacting motif 1) via a novel MIM-binding structural motif. PMID: 19477918
  3. We show that Did2 directs Vps4 activity to the dissociation of ESCRT-III but has no role in the dissociation of ESCRT-I or -II. PMID: 17130288
  4. A model in which the Ist1-Did2 and Vta1-Vps60 complexes independently modulate late steps in the multivesicular body-sorting pathway, is proposed. PMID: 18032584
Subcellular Location
Endosome membrane; Peripheral membrane protein. Endomembrane system; Peripheral membrane protein. Note=Endosomal and other punctate structures.
Protein Families
SNF7 family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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