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Recombinant Saccharomyces cerevisiae mRNA-decapping enzyme subunit 2 (DCP2), partial

Recombinant Saccharomyces cerevisiae mRNA-decapping enzyme subunit 2 (DCP2), partial

Code	CSB-YP347214SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP347214SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP347214SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP347214SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP347214SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

DCP2

Uniprot No.

P53550

Alternative Names

DCP2; PSU1; YNL118C; N1917m7GpppN-mRNA hydrolase; EC 3.6.1.62; Protein PSU1; mRNA-decapping enzyme subunit 2

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Catalytic component of the decapping complex necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Decapping is the major pathway of mRNA degradation in yeast and occurs through deadenylation, decapping and subsequent 5' to 3' exonucleolytic decay of the transcript body. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.

Gene References into Functions

This recruitment requires Hrr25 kinase activity and the Dcp2 decapping enzyme, a core constituent of these RNP granules. Interestingly, the data indicate that this localization sequesters active Hrr25 away from the remainder of the cytoplasm and thereby shields this enzyme from the degradation machinery during these periods of stress. PMID: 27182950
Results show that C-terminal domain of Dcp2 includes a set of conserved negative and positive regulatory elements reveal a pivotal role for the Dcp2 in mRNA decapping and lead to a new model for decapping enzyme regulation in vivo. PMID: 26184073
Local active site conformational changes are important for Dcp2 mRNA decapping activity. PMID: 23911090
show using NMR that cap binds conserved residues on both the catalytic and regulatory domains of Dcp2. PMID: 20711189
Ste20 has an unexpected role in the modulation of mRNA decay and translation in Saccharomyces cerevisiae. Phosphorylation of Dcp2 by Ste20 is an important control point for mRNA decapping. PMID: 20513766
These results raise a model for Dcp2 recruitment to specific mRNAs where regions outside the catalytic core promote the formation of different complexes involved in mRNA decapping. PMID: 20086104
To understand regulation of Dcp2 by ligand interactions, we have assigned the backbone and sidechain methyl Ile (delta1), Leu and Val chemical shifts of the catalytic domain. PMID: 19636815