Recombinant Saccharomyces cerevisiae mRNA decay factor CTH2 (TIS11)

Code CSB-YP343701SVG
MSDS
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Source Yeast
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Code CSB-EP343701SVG
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Source E.coli
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Code CSB-EP343701SVG-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP343701SVG
MSDS
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Source Baculovirus
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Code CSB-MP343701SVG
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TIS11
Uniprot No.
Alternative Names
TIS11; CTH2; YLR136C; L3143; L9606.12mRNA decay factor CTH2; Cysteine-three-histidine protein 2; Protein TIS11 homolog; Protein YTIS11; TPA-induced sequence protein 11
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-285
Target Protein Sequence
MWAQLSYTRP ESQKTDLTSL FSTDQEQNPL NDYQYQINIR ELEEYYNKTI LNEDNIQETS SEISSAVSFS PPKNTNAIQP GLLYDPQLMN PFLPSAHLNS TAPTTFKKKL EVQINPDYVP KSSQLPLTSQ NLQQLSQQKP KNDASFSSEK ESSAQPKVKS QVQETPKQLY KTELCESFTL KGSCPYGSKC QFAHGLGELK VKKSCKNFRT KPCVNWEKLG YCPYGRRCCF KHGDDNDIAV YVKAGTYCNV SSTSKQSDEK RSNGRGSAKK KNLNVKVKAL QRMTW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds to specific AU-rich elements (ARE) in the 3'-untranslated region of target mRNAs and promotes their degradation. In response to iron deficiency, promotes the decay of many mRNAs encoding proteins involved in iron-dependent pathways. Recruits the DHH1 helicase to the SDH4 mRNA and promotes SDH4 mRNA decay. Also destabilizes target mRNA by modulating 3'-end processing, creating extended transcripts that are prone for degradation.
Gene References into Functions
  1. Results demonstrate that the Cth2 CTD is physiologically relevant for adaptation to iron deficiency. PMID: 29912874
  2. Cth2 protein mediates early adaptation of yeast cells to oxidative stress conditions. PMID: 26824473
  3. Negative feedback regulation of the CTH1 and CTH2 mRNA binding proteins is required for adaptation to iron deficiency and iron supplementation. PMID: 23530061
  4. metabolic reprogramming as a consequence of Fe starvation that is dependent on the coordinated activities of CTH1 and CTH2 PMID: 18522836
  5. Cth2 protein recruits the Dhh1 helicase to ARE-containing mRNAs to promote SDH4 mRNA decay PMID: 18715869

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Subcellular Location
Nucleus. Cytoplasm, P-body.
Database Links

KEGG: sce:YLR136C

STRING: 4932.YLR136C

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