Recombinant Saccharomyces cerevisiae rRNA-processing protein EBP2 (EBP2)

Code CSB-YP330525SVG
MSDS
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Source Yeast
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Code CSB-EP330525SVG
MSDS
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Source E.coli
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Code CSB-EP330525SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP330525SVG
MSDS
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Source Baculovirus
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Code CSB-MP330525SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EBP2
Uniprot No.
Alternative Names
EBP2; YKL172W; YKL636rRNA-processing protein EBP2; EBNA1-binding protein homolog
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-427
Target Protein Sequence
MAKGFKLKEL LSHQKEIEKA EKLENDLKKK KSQELKKEEP TIVTASNLKK LEKKEKKADV KKEVAADTEE YQSQALSKKE KRKLKKELKK MQEQDATEAQ KHMSGDEDES GDDREEEEEE EEEEEGRLDL EKLAKSDSES EDDSESENDS EEDEDVVAKE ESEEKEEQEE EQDVPLSDVE FDSDADVVPH HKLTVNNTKA MKHALERVQL PWKKHSFQEH QSVTSETNTD EHIKDIYDDT ERELAFYKQS LDAVLVARDE LKRLKVPFKR PLDYFAEMVK SDEHMDKIKG KLIEEASDKK AREEARRQRQ LKKFGKQVQN ATLQKRQLEK RETLEKIKSL KNKRKHNEID HSEFNVGVEE EVEGKRFDRG RPNGKRAAKN AKYGQGGMKR FKRKNDATSS ADVSGFSSRK MKGKTNRPGK SRRARRF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for the processing of the 27S pre-rRNA. Probably involved in the step of the processing of the 27 SA precursor into the 27 SB intermediate.
Gene References into Functions
  1. Depletion of either Ebp2 or Brx1 revealed that Brx1 requires Ebp2 for its stable association with pre-ribosomes, but Ebp2 does not depend on the presence of Brx1 to enter pre-ribosomes. PMID: 22319211
  2. plays a role in nuclear division in yeast and this function is separable from the role it plays in rRNA biosynthesis. PMID: 15515129
  3. Ebp2p is implicated in the secretory response, including changes in nucleolar architecture PMID: 18391458
  4. SUMO mediates the interaction between Ebp2p and SUMO related proteins and Ebp2p switches its interaction partners via sumoylation. PMID: 18603780
Subcellular Location
Nucleus, nucleolus.
Protein Families
EBP2 family
Database Links

KEGG: sce:YKL172W

STRING: 4932.YKL172W

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