Recombinant Saponaria officinalis Ribosome-inactivating protein saporin-9 (SAP9)

Product Details

Purity

>85% (SDS-PAGE)

Target Names

SAP9

Uniprot No.

Q7M1Z2

Alternative Names

SAP9; Ribosome-inactivating protein saporin-9; SAP-9; SO-9; EC 3.2.2.22; rRNA N-glycosidase

Species

Saponaria officinalis (Common soapwort) (Lychnis saponaria)

Expression Region

1-253

Target Protein Sequence

VTSITLDLVN PTAGQYSSFV DKIRNNVKDP NLKYGGTDIA VIGPPSKDKF LRINFQSSRG TVSLGLKRDN LYVVAYLAMD NTNVNRAYYF RSEITSAELT ALFPEATAAN HKALEYTEDY HSIEKNAQIT EGDKSRKELG LGINLLSSTM DTVNKKVRVV KNEARFLLIA IQMTAEAVRF RYIQNLVTKN FPNKFNSENK VIKFEVNWKK ISTAIHGDAK NGVFNKDYDF GFGKVRLVKD LQMGLLMHLG KPK

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

Could you share what kind of yeast you use to produce saporin 9 (CSB-YP748739SCG)?

The strain that we use for Yeast expression system is Pichia pastoris.

During the process, does your company optimize Yeast Condon?

Yes, we do.

Could you share nucleotide sequence information for this protein (CSB-YP748739SCG)?

GTTACCTCCATCACCTTGGACTTGGTTAACCCAACTGCTGGTCAGTACTCCTCATTCGTCGACAAGATCAGAAACAACGTCAAGGACCCAAACCTGAAGTACGGTGGTACTGACATTGCTGTTATTGGTCCACCATCCAAGGACAAGTTCTTGAGAATCAACTTCCAGTCCTCCAGAGGTACTGTCTCCTTGGGTTTGAAGAGAGACAACTTGTACGTCGTTGCCTACTTGGCTATGGACAACACCAACGTTAACAGAGCCTACTACTTCAGATCCGAGATCACTTCCGCTGAGTTGACTGCTTTGTTCCCAGAAGCTACTGCTGCTAACCATAAGGCTTTGGAGTACACTGAGGACTACCACTCCATTGAGAAGAACGCTCAGATTACCGAAGGTGACAAGTCCAGAAAAGAGCTTGGTCTGGGTATCAACTTGTTGTCCTCCACTATGGACACCGTCAACAAGAAGGTTAGAGTCGTTAAGAACGAGGCCAGGTTCTTGCTGATTGCTATCCAAATGACTGCTGAGGCCGTCAGATTCAGGTACATCCAGAACTTGGTCACCAAGAACTTCCCCAACAAGTTCAACTCCGAGAACAAGGTCATCAAGTTCGAGGTCAACTGGAAGAAGATCTCCACTGCTATTCACGGTGACGCCAAGAACGGTGTTTTCAACAAGGATTACGACTTCGGTTTCGGTAAGGTCAGACTGGTTAAGGACTTGCAGATGGGTTTGTTGATGCACTTGGGTAAGCCAAAG

After generating the protein (CSB-YP748739SCG), does your company confirm the sequence of every protein using Sanger or other methods?

Yes, after the construction of expression plasmid, sequencing will be performed to ensure that the nucleotide sequence is consistent with the designed sequence.

Could you share the plasmids of the items with us?

The plasmids of the original Saporin9 is not for sale. Since the sequence-modified ones are custom proteins for you, if you order 1mg of each proteins, we can provide the plasmids along with the Concluding Report, but there will be extra cost.

I was wondering if you could ship it to us in liquid form after purification. Do NOT lyophilize because we are concerned that drying the protein affects its functionality. Is it active form of toxin?

Yes, we can ship this protein in liquid form. We haven't expressed this protein yet, its activity is not guaranteed. In theory, this is a full length protein and we adopt affinity chromatography for purification, which is purified under a very mild condition, it's very likely to be active.

Target Background

Function

Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells.

Protein Families

Ribosome-inactivating protein family, Type 1 RIP subfamily

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Recombinant Saponaria officinalis Ribosome-inactivating protein saporin-9 (SAP9)

Product Details

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Customer Reviews and Q&A

Target Background

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