Recombinant Scheffersomyces stipitis NAD (P)H-dependent D-xylose reductase

Code CSB-YP330121SXM
MSDS
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Source Yeast
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Code CSB-EP330121SXM
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Source E.coli
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Code CSB-EP330121SXM-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP330121SXM
MSDS
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Source Baculovirus
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Code CSB-MP330121SXM
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
XYL1
Uniprot No.
Alternative Names
XYL1; PICST_89614NAD(P)H-dependent D-xylose reductase; XR; EC 1.1.1.307
Species
Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545) (Yeast) (Pichia stipitis)
Expression Region
1-318
Target Protein Sequence
MPSIKLNSGY DMPAVGFGCW KVDVDTCSEQ IYRAIKTGYR LFDGAEDYAN EKLVGAGVKK AIDEGIVKRE DLFLTSKLWN NYHHPDNVEK ALNRTLSDLQ VDYVDLFLIH FPVTFKFVPL EEKYPPGFYC GKGDNFDYED VPILETWKAL EKLVKAGKIR SIGVSNFPGA LLLDLLRGAT IKPSVLQVEH HPYLQQPRLI EFAQSRGIAV TAYSSFGPQS FVELNQGRAL NTSPLFENET IKAIAAKHGK SPAQVLLRWS SQRGIAIIPK SNTVPRLLEN KDVNSFDLDE QDFADIAKLD INLRFNDPWD WDKIPIFV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Reduces D-xylose into xylitol. Has a preference for NADPH, but can also utilize NADH as cosubstrate.
Gene References into Functions
  1. The 3D structure of NAD(P)H-dependent D-xylose reductase was modeled, and the binding pockets of the enzyme for its ligands NAD and NADP were defined. [XYLO] PMID: 17544374
  2. Introduced in S.cerevisiae F12, succesfully enable it to metabolize xylose from cereal straw PMID: 18383076
Protein Families
Aldo/keto reductase family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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